Li Zhiwen, Liu Yang, Liu Gaofeng, Zhu Junfei, Zheng Zhibing, Zhou Ying, He Junlin
College of Life Sciences, Guizhou University, Guiyang 550025, China.
School of Pharmacological Sciences, Guangxi Medical University, Nanning 530021, China.
Bioorg Med Chem. 2014 Aug 1;22(15):4010-7. doi: 10.1016/j.bmc.2014.05.070. Epub 2014 Jun 9.
Nucleoside analogues with imidazolyl and histidinyl groups were synthesized for site-specific modification on the catalytic core of 10-23 DNAzyme. The distinct position-dependent effect of imidazolyl group was observed. Positive effect at A9 position was always observed. The pH- and Mg(2+)-dependence of the imidazolyl-modified DNAzymes suggested that imidazolyl group in 10-23 DNAzyme probably plays a dual role, its hydrogen bonding ability and spacial occupation play the favorable influence on the catalytic conformation of the modified DNAzymes. This research demonstrated that the catalytic performance of DNAzymes could be enhanced by incorporation of additional functional groups. Chemical modification is a feasible approach toward more efficient DNAzymes for therapeutic and biotechnological applications.
合成了带有咪唑基和组氨酸基的核苷类似物,用于对10-23脱氧核酶的催化核心进行位点特异性修饰。观察到咪唑基有明显的位置依赖性效应。在A9位置总是观察到正向效应。咪唑基修饰的脱氧核酶对pH和Mg(2+)的依赖性表明,10-23脱氧核酶中的咪唑基可能起双重作用,其氢键结合能力和空间占据对修饰后的脱氧核酶的催化构象产生有利影响。本研究表明,通过引入额外的官能团可以提高脱氧核酶的催化性能。化学修饰是一种可行的方法,可用于制备更高效的脱氧核酶以用于治疗和生物技术应用。