通过重新研究 10-23 DNA 酶的催化核心，发现了一种新的 Pb 特异性 DNA 酶。
A new Pb-specific DNAzyme by revisiting the catalytic core of 10-23 DNAzyme.
机构信息
School of Life Science and Engineering, Handan University, Handan 056005, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; Hebei Key Laboratory of Heterocyclic Compounds, College of Chemical Engineering and Materials, Handan University, Handan 056005, China.
出版信息
Bioorg Med Chem. 2020 Nov 15;28(22):115796. doi: 10.1016/j.bmc.2020.115796. Epub 2020 Oct 1.
10-23 DNAzyme is a catalytic DNA molecule from in vitro selection, the 15-mer catalytic core was investigated for more DNAzyme variants by block deletions. DNAzyme DZM01 was selected with metal ion dependence of Pb ≫ Mn, with no activity in the presence of Mg (20 mM), Ca (20 mM), Zn (20 mM, pH 6). The unique binding properties of Pb with nucleic acids might be responsible for the formation of the catalytic core, which is different from that of other divalent metal ions. More DNAzyme variants are expected to be derived for specific metal ion dependence by various nucleobase sequences and modifications.
10-23 DNAzyme 是一种经体外筛选得到的催化型 DNA 分子，通过阻断缺失法对 15 -mer 催化核心进行了更多 DNAzyme 变体的研究。DNAzyme DZM01 的筛选依赖于 Pb > Mn 的金属离子，在存在 Mg（20 mM）、Ca（20 mM）、Zn（20 mM，pH 6）的情况下没有活性。Pb 与核酸的独特结合特性可能是形成催化核心的原因，这与其他二价金属离子不同。通过各种碱基序列和修饰，预计会衍生出更多具有特定金属离子依赖性的 DNAzyme 变体。
Zotero 插件