Kaliňák Michal, Simkovič Martin, Zemla Peter, Matata Matej, Molnár Tomáš, Liptaj Tibor, Varečka L'udovít, Hudecová Daniela
Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
FEMS Microbiol Lett. 2014 Aug;357(2):201-7. doi: 10.1111/1574-6968.12516. Epub 2014 Jul 21.
The aim of this work was to study the metabolic changes during germination of Trichoderma atroviride conidia along with selected marker enzyme activities. The increase in proteinogenic amino acid concentrations together with the increase in glutamate dehydrogenase activity suggests a requirement for nitrogen metabolism. Even though the activities of tricarboxylic acid cycle enzymes also increased, detected organic acid pools did not change, which predisposes this pathway to energy production and supply of intermediates for further metabolism. The concentrations of many metabolites, including the main osmolytes mannitol and betaine, also increased during the formation of germ tubes. The activities of H(+)-ATPase and GDPase, the only marker enzymes that did not have detectable activity in non-germinated conidia, were shown with germ tubes.
这项工作的目的是研究绿色木霉分生孢子萌发过程中的代谢变化以及选定的标记酶活性。蛋白质氨基酸浓度的增加以及谷氨酸脱氢酶活性的增加表明对氮代谢有需求。尽管三羧酸循环酶的活性也增加了，但检测到的有机酸池没有变化，这使得该途径倾向于能量产生以及为进一步代谢提供中间体。在芽管形成过程中，包括主要渗透调节物质甘露醇和甜菜碱在内的许多代谢物浓度也增加了。H(+)-ATP酶和GDP酶是仅有的在未萌发分生孢子中没有可检测活性的标记酶，它们在芽管中表现出活性。
