Papadopoulou Anastasia, Gerdemann Ulrike, Katari Usha L, Tzannou Ifigenia, Liu Hao, Martinez Caridad, Leung Kathryn, Carrum George, Gee Adrian P, Vera Juan F, Krance Robert A, Brenner Malcolm K, Rooney Cliona M, Heslop Helen E, Leen Ann M
Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital, Houston Methodist Hospital, Houston, TX 77030, USA.
Sci Transl Med. 2014 Jun 25;6(242):242ra83. doi: 10.1126/scitranslmed.3008825.
It remains difficult to treat the multiplicity of distinct viral infections that afflict immunocompromised patients. Adoptive transfer of virus-specific T cells (VSTs) can be safe and effective, but such cells have been complex to prepare and limited in antiviral range. We now demonstrate the feasibility and clinical utility of rapidly generated single-culture VSTs that recognize 12 immunogenic antigens from five viruses (Epstein-Barr virus, adenovirus, cytomegalovirus, BK virus, and human herpesvirus 6) that frequently cause disease in immunocompromised patients. When administered to 11 recipients of allogeneic transplants, 8 of whom had up to four active infections with the targeted viruses, these VSTs proved safe in all subjects and produced an overall 94% virological and clinical response rate that was sustained long-term.
治疗免疫功能低下患者所患的多种不同病毒感染仍然很困难。病毒特异性T细胞（VSTs）的过继转移可能是安全有效的，但此类细胞制备复杂且抗病毒范围有限。我们现在证明了快速生成的单培养VSTs的可行性和临床实用性，这些VSTs可识别五种病毒（爱泼斯坦-巴尔病毒、腺病毒、巨细胞病毒、BK病毒和人类疱疹病毒6）的12种免疫原性抗原，这些病毒经常在免疫功能低下的患者中引发疾病。当将这些VSTs给予11名同种异体移植受者时，其中8人感染了多达四种靶向病毒，结果证明这些VSTs对所有受试者都是安全的，并产生了94%的总体病毒学和临床反应率，且这种反应率能长期维持。
