Dettmer Katja
Institute of Functional Genomics, University of Regensburg, Josef-Engert-Str. 9, 93053, Regensburg, Germany,
Anal Bioanal Chem. 2014 Aug;406(20):4931-9. doi: 10.1007/s00216-014-7919-0. Epub 2014 Jun 26.
The gas chromatographic separation of fatty acid methyl esters (FAMEs) on ionic liquid stationary phases was investigated. Seven commercially available ionic liquid columns were tested using a test mixture containing 37 fatty acid methyl esters. The influence of column temperature on the elution order was studied using five different temperature programs. Retention times were highly reproducible. Similar retention behavior was observed for the IL59, IL60, and IL61 columns. The peak pair C18:1 cis/trans was not baseline resolved on these columns, whose stationary phases are highly similar. C18:2 cis/trans, C18:3 n6/n3, and C20:3 n6/n3 were baseline separated on all columns. Baseline separation of the complete test mix was only obtained on the IL82 column using a heating rate of 5 K/min. In general, retention times decreased with increasing column polarity but unsaturated FAMEs were retained stronger compared to their saturated counterparts. Except for the IL59 column, retention crossover was observed when the temperature program was changed.
研究了脂肪酸甲酯（FAMEs）在离子液体固定相上的气相色谱分离。使用含有37种脂肪酸甲酯的测试混合物对7种市售离子液体柱进行了测试。使用5种不同的温度程序研究了柱温对洗脱顺序的影响。保留时间具有高度可重复性。IL59、IL60和IL61柱观察到相似的保留行为。在这些固定相高度相似的柱上，峰对C18:1顺式/反式未实现基线分离。C18:2顺式/反式、C18:3 n6/n3和C20:3 n6/n3在所有柱上均实现了基线分离。仅在IL82柱上以5 K/min的升温速率获得了完整测试混合物的基线分离。一般来说，保留时间随柱极性的增加而降低，但不饱和脂肪酸甲酯比其饱和对应物保留得更强。除IL59柱外，当改变温度程序时观察到保留交叉现象。
