Differences in response of acetylcholinesterase to diisopropylfluorophosphate in the mesencephalic raphe region. A microelectrophoretic, histochemical, and biochemical study in albino rats.

To demonstrate the DFP-effects on the AChE of the mesencephalic raphe region albino rats were investigated using the pharmaco-histochemical method of Butcher et al. (1975). At several times after 1 mg DFP/kg body weight, the histochemical reactions in certain AChE-containing neurons were measured semiquantitatively and compared with biochemical data. Additionally, the multiple forms of water-soluble AChE were separated by microelectrophoresis. Only the electrophoretical results indicate changes of the structural AChE composition 5 d after DFP administration.