Chen Jun, Xiao Hengjun, Huang Zhansen, Hu Zhiming, Qi Tao, Zhang Bin, Tao Xin, Liu Song-Hao
Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University Guangzhou 510006, P. R. China ; Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630, P. R. China.
Int J Clin Exp Pathol. 2014 Apr 15;7(5):2283-90. eCollection 2014.
Protein phosphatase 1, regulatory subunit 13 like PPP1R13L, also coined iASPP, was found high expression in prostate cancer tissues and cell lines. In previous research, in vitro and in vivo RNAi mediated by artificial lentiviral shRNAs which proved that suppression of iASPP decrease the proliferation of cancer cells. Endogenous interference RNAs, microRNAs play key roles in cell proliferation by post-transcriptional regulation of gene expression. Natural base pair matched microRNA for iASPP is mir124, which was found high expression in growth factorloss prostate cancer cell lines. In this study we examined effect of mir124 upon iASPP and proliferation of prostate cells in vitro with lentiviral infection and use artificial shRNA as control. In vitro reporter assay confirmed that mir124 binding the 3'UTR of iASPP and suppress mRNA expression. Lentivirus mediated mir124 expression decreased the proliferation and viability of PC3 while endogenous iASPP were knocked down.
蛋白磷酸酶1调节亚基13样蛋白PPP1R13L，也被称为iASPP，在前列腺癌组织和细胞系中高表达。在先前的研究中，人工慢病毒短发夹RNA介导的体外和体内RNA干扰证明，抑制iASPP可降低癌细胞的增殖。内源性干扰RNA，即微小RNA，通过对基因表达的转录后调控在细胞增殖中起关键作用。与iASPP天然碱基对匹配的微小RNA是mir124，其在生长因子缺失的前列腺癌细胞系中高表达。在本研究中，我们通过慢病毒感染检测了mir124对体外iASPP和前列腺细胞增殖的影响，并以人工短发夹RNA作为对照。体外报告基因检测证实mir124与iASPP的3'非翻译区结合并抑制mRNA表达。慢病毒介导的mir124表达降低了PC3的增殖和活力，同时内源性iASPP被敲低。
