Liu Ze-Jun, Cai Yun, Hou Lu, Gao Xing, Xin Hai-Ming, Lu Xin, Zhong Shan, Gu Shou-Zhi, Chen Jie
Laboratory of International Cooperation, Southwest Hospital, Third Military Medical University, Chongqing, PRC.
Cancer Invest. 2008 Nov;26(9):878-82. doi: 10.1080/07357900801965042.
ASPP family is proved to be apoptotic specific regulators of p53. Among them, iASPP acts as an inhibitor of p53. To investigate the effect of the iASPP RNAi on the apoptosis of breast cancer cell MCF-7, we transfected the recombinant plasmid PGCsilencer H1/Neo/GFP/RNAi into MCF-7. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. The results show that the expression of iASPP is descended and the apoptosis rate is increased after transfection. Therefore, we conclude that inhibition of expression of iASPP may resume the ability of p53 to induce apoptosis in MCF-7 cells.
ASPP家族被证明是p53的凋亡特异性调节因子。其中，iASPP作为p53的抑制剂。为了研究iASPP RNA干扰对乳腺癌细胞MCF-7凋亡的影响，我们将重组质粒PGCsilencer H1/Neo/GFP/RNAi转染到MCF-7中。通过RT-PCR和蛋白质免疫印迹分析iASPP的表达。通过流式细胞术检测细胞凋亡。结果显示，转染后iASPP的表达下降，凋亡率增加。因此，我们得出结论，抑制iASPP的表达可能恢复p53诱导MCF-7细胞凋亡的能力。
