Infectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Iran J Basic Med Sci. 2014 May;17(5):370-7.
It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients.
In this study, 120 chronic HBV patients from Golestan, Northeast of Iran who were not vaccinated against HBV, were recruited from the year 2008 to 2012. HBV-DNA extraction from plasma and PCR were performed and positive PCR products were subjected to automated sequencing.
One hundred out of 120 (83.3%) patients were HBeAg negative. Comparison of our nucleotide sequences with reference sequence showed high rate mutation in BCP and PC region (96.66%). Frame shift mutation was found in 78 (65%) of patients in BCP region, among them 8 (6.6%) patients showed mutation in PC region.
Our results demonstrated high rate of mutations in BCP and PC regions among HBV chronic patients in Northeast of Iran.
据报道，前核心（PC）和基本核心启动子（BCP）的突变可能在乙型肝炎病毒（HBV）相关肝细胞癌（HCC）的发展中起重要作用。本研究调查了慢性 HBV 患者中 PC 和 BCP 的突变情况。
本研究纳入了 2008 年至 2012 年来自伊朗东北部戈勒斯坦的 120 名未接种 HBV 的慢性 HBV 患者。从血浆中提取 HBV-DNA 并进行 PCR，对阳性 PCR 产物进行自动测序。
120 例患者中有 100 例（83.3%）HBeAg 阴性。将我们的核苷酸序列与参考序列进行比较，发现 BCP 和 PC 区域的突变率很高（96.66%）。在 BCP 区域中，78 例（65%）患者发现了移码突变，其中 8 例（6.6%）患者在 PC 区域发生了突变。
我们的结果表明，伊朗东北部慢性 HBV 患者的 BCP 和 PC 区域存在高突变率。
