Punnonen J, Viljanen M K
Department of Medical Microbiology, University of Turku, Finland.
APMIS. 1989 Mar;97(3):200-6. doi: 10.1111/j.1699-0463.1989.tb00778.x.
We studied the effects of recombinant interleukin 2 (rIL 2), interferon-alpha (rIFN-alpha) and interferon-gamma (rIFN-gamma) on proliferation and IgM synthesis of highly purified tonsillar B cells either without in vitro preactivation or after activation with Staphylococcus aureus Cowan 1 (SAC). Only rIL 2 affected tonsillar B cells not preactivated in vitro. It induced both proliferation and differentiation of B cells in a dose-dependent manner. rIL 2 induced proliferation of even Tac-negative B cells, but statistically significant responses were obtained only at high concentrations. The response was found also when no serum supplement was used in the culture medium. The finding suggests that tonsillar B cells express IL 2 receptors distinct from Tac antigen. When the cells were activated with SAC, significant proliferation was also found in response to rIFN-gamma. However, rIFN-gamma induced no IgM synthesis even after activation with SAC. rIFN-alpha affected neither B cell proliferation nor differentiation regardless of whether the cells were activated with SAC or not. Our results suggest that IL 2 can induce proliferation of activated B cells, but IFN-gamma only enhances proliferation of actively cycling B cells.
我们研究了重组白细胞介素2（rIL 2）、α干扰素（rIFN-α）和γ干扰素（rIFN-γ）对高度纯化的扁桃体B细胞增殖及IgM合成的影响，这些B细胞要么未经过体外预激活，要么已被 Cowan 1 型金黄色葡萄球菌（SAC）激活。只有rIL 2对未在体外预激活的扁桃体B细胞有影响。它以剂量依赖的方式诱导B细胞增殖和分化。rIL 2甚至能诱导Tac阴性B细胞增殖，但只有在高浓度时才能获得统计学上显著的反应。当培养基中不添加血清时也能观察到这种反应。这一发现表明扁桃体B细胞表达不同于Tac抗原的IL 2受体。当细胞被SAC激活后，对rIFN-γ也有显著的增殖反应。然而，即使在被SAC激活后，rIFN-γ也不诱导IgM合成。无论细胞是否被SAC激活，rIFN-α对B细胞增殖和分化均无影响。我们的结果表明，IL 2能诱导活化B细胞增殖，而IFN-γ仅增强活跃循环的B细胞的增殖。
