Camassola Marli, Dillon Aldo J P
Institute of Biotechnology, University of Caxias do Sul, P.O. Box 1352, 95070-560 Caxias do Sul, RS, Brazil.
Biomed Res Int. 2014;2014:720740. doi: 10.1155/2014/720740. Epub 2014 May 20.
The main limitation to the industrial scale hydrolysis of cellulose is the cost of cellulase production. This study evaluated cellulase and xylanase enzyme production by the cellulolytic mutant Penicillium echinulatum 9A02S1 using pretreated sugar cane bagasse as a carbon source. Most cultures grown with pretreated bagasse showed similar enzymatic activities to or higher enzymatic activities than cultures grown with cellulose or untreated sugar cane bagasse. Higher filter paper activity (1.253 ± 0.147 U · mL(-1)) was detected in the medium on the sixth day of cultivation when bagasse samples were pretreated with sodium hydroxide, hydrogen peroxide, and anthraquinone. Endoglucanase enzyme production was also enhanced by pretreatment of the bagasse. Nine cultures grown with bagasse possessed higher β -glucosidase activities on the sixth day than the culture grown with cellulose. The highest xylanase activity was observed in cultures with cellulose and with untreated sugar cane bagasse. These results indicate that pretreated sugar cane bagasse may be able to serve as a partial or total replacement for cellulose in submerged fermentation for cellulase production using P. echinulatum, which could potentially reduce future production costs of enzymatic complexes capable of hydrolyzing lignocellulosic residues to form fermented syrups.
纤维素进行工业规模水解的主要限制在于纤维素酶的生产成本。本研究评估了纤维素分解突变体棘孢青霉9A02S1以预处理甘蔗渣作为碳源生产纤维素酶和木聚糖酶的情况。大多数用预处理甘蔗渣培养的菌株表现出与用纤维素或未处理甘蔗渣培养的菌株相似或更高的酶活性。当甘蔗渣样品用氢氧化钠、过氧化氢和蒽醌预处理时，在培养的第六天，培养基中检测到较高的滤纸酶活性（1.253±0.147 U·mL(-1)）。甘蔗渣的预处理也提高了内切葡聚糖酶的产量。九个用甘蔗渣培养的菌株在第六天的β-葡萄糖苷酶活性高于用纤维素培养的菌株。在以纤维素和未处理甘蔗渣培养的菌株中观察到最高的木聚糖酶活性。这些结果表明，预处理甘蔗渣在使用棘孢青霉进行纤维素酶生产的深层发酵中，可能能够部分或全部替代纤维素，这有可能降低未来能够水解木质纤维素残渣以形成发酵糖浆的酶复合物的生产成本。
