Reis Leonardo F, Minervino Antonio H H, Araújo Carolina A S C, Sousa Rejane S, Oliveira Francisco L C, Rodrigues Frederico A M L, Meira-Júnior Enoch B S, Barrêto-Júnior Raimundo A, Mori Clara S, Ortolani Enrico L
Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
Institute of Biodiversity and Forest, Federal University of Western Pará, Avenida Vera Paz S/N, Salé, 68000-000 Santarém, PA, Brazil.
ScientificWorldJournal. 2014;2014:195782. doi: 10.1155/2014/195782. Epub 2014 May 21.
We aimed to compare the measurements of sheep ruminal pH using a continuous telemetry system or a bench pH meter using sheep with different degrees of ruminal pH. Ruminal lactic acidosis was induced in nine adult crossbred Santa Ines sheep by the administration of 15 g of sucrose per kg/BW. Samples of rumen fluid were collected at the baseline, before the induction of acidosis (T 0) and at six, 12, 18, 24, 48, and 72 hours after the induction for pH measurement using a bench pH meter. During this 72-hour period, all animals had electrodes for the continuous measurement of pH. The results were compared using the Bland-Altman analysis of agreement, Pearson coefficients of correlation and determination, and paired analysis of variance with Student's t-test. The measurement methods presented a strong correlation (r = 0.94, P < 0.05) but the rumen pH that was measured continuously using a telemetry system resulted in lower values than the bench pH meter (overall mean of 5.38 and 5.48, resp., P = 0.0001). The telemetry system was able to detect smaller changes in rumen fluid pH and was more accurate in diagnosing both subacute ruminal lactic acidosis and acute ruminal lactic acidosis in sheep.
我们旨在比较使用连续遥测系统或台式pH计对不同瘤胃pH程度的绵羊进行瘤胃pH测量的结果。通过每千克体重给予15克蔗糖，在9只成年圣伊内斯杂交绵羊中诱发瘤胃酸中毒。在酸中毒诱导前的基线（T 0）以及诱导后的6、12、18、24、48和72小时采集瘤胃液样本，使用台式pH计测量pH。在这72小时期间，所有动物都安装了用于连续测量pH的电极。使用Bland-Altman一致性分析、Pearson相关系数和决定系数以及配对方差分析和Student t检验对结果进行比较。测量方法呈现出很强的相关性（r = 0.94，P < 0.05），但使用遥测系统连续测量的瘤胃pH值低于台式pH计（分别为5.38和5.48的总体平均值，P = 0.0001）。遥测系统能够检测到瘤胃液pH的较小变化，并且在诊断绵羊亚急性瘤胃酸中毒和急性瘤胃酸中毒方面更准确。
