Hong Cheol Am, Jang Bora, Jeong Eun Hye, Jeong Hansaem, Lee Hyukjin
College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
Chem Commun (Camb). 2014 Nov 7;50(86):13049-51. doi: 10.1039/c4cc03834g.
Inspired by the isothermal enzymatic process of rolling circle amplification (RCA) of DNA strands, we have developed a system to achieve more than a 200-fold increase in the synthesis of DNA nanostructures using a single-stranded circular DNA template. The amplified DNA nanostructures have shown efficient delivery of folic acid (FA) conjugated siRNAs into KB cells with a dose dependent gene silencing.
受DNA链滚环扩增（RCA）等温酶促过程的启发，我们开发了一种系统，该系统使用单链环状DNA模板，可使DNA纳米结构的合成增加200倍以上。扩增后的DNA纳米结构已显示出将叶酸（FA）偶联的小干扰RNA（siRNA）有效递送至KB细胞，并具有剂量依赖性的基因沉默作用。
