通过滚环扩增制备的用于递送小干扰RNA缀合物的自组装DNA纳米结构。

Self-assembled DNA nanostructures prepared by rolling circle amplification for the delivery of siRNA conjugates.

作者信息

Hong Cheol Am, Jang Bora, Jeong Eun Hye, Jeong Hansaem, Lee Hyukjin

机构信息

College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.

出版信息

Chem Commun (Camb). 2014 Nov 7;50(86):13049-51. doi: 10.1039/c4cc03834g.

DOI:10.1039/c4cc03834g

PMID:24967959

Abstract

Inspired by the isothermal enzymatic process of rolling circle amplification (RCA) of DNA strands, we have developed a system to achieve more than a 200-fold increase in the synthesis of DNA nanostructures using a single-stranded circular DNA template. The amplified DNA nanostructures have shown efficient delivery of folic acid (FA) conjugated siRNAs into KB cells with a dose dependent gene silencing.

摘要

受DNA链滚环扩增（RCA）等温酶促过程的启发，我们开发了一种系统，该系统使用单链环状DNA模板，可使DNA纳米结构的合成增加200倍以上。扩增后的DNA纳米结构已显示出将叶酸（FA）偶联的小干扰RNA（siRNA）有效递送至KB细胞，并具有剂量依赖性的基因沉默作用。