Karimi Mohammad Hossein, Salek Sanaz, Yaghobi Ramin, Ramzi Mani, Geramizadeh Bita, Hejr Sara
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Cytokine. 2014 Sep;69(1):120-4. doi: 10.1016/j.cyto.2014.05.011. Epub 2014 Jun 16.
Cytokines are important factors determining the outcome of transplantation. Host ability in cytokine production may be affected by cytokine genes polymorphisms. The aim of the present study was to investigate the effect of IL-17 gene polymorphisms on outcome of Hematopoietic stem cell transplantation.
A total of 60 bone marrow recipients were included in this study. Twenty-five recipients (41.66%) underwent a GVHD. IL-17 gene polymorphisms were evaluated by PCR-RFLP method and the serum levels were also checked by ELISA.
No significant differences in distribution of the IL-17(A/G) (rs3819025) genotypes and alleles were observed between two groups. But, IL-17 (A/G, -197) GG genotype was found to be significantly higher in GVHD group compared to those of non-GVHD group (P = 0.04). Interestingly, after stratification of patients according severity of GVHD, IL-17 (rs3819025) G allele was remained significantly higher in GVHD grade (0-I) group compared to those of grade (II-IV) group (P = 0.05). In addition, after categorization of patients according to their sex, IL-17-197 GG genotype showed a significant association with non-GVHD in male patients (P = 0.05). IL-17 serum levels did not show any significant difference between GVHD and non GVHD groups.
Results indicated that IL-17197 GG genotype, G allele of (rs3819025) and its serum level have predictive values for severity of GVHD. Also, IL-17-197 GG genotype is a sex dependent genetic risk factor for development of GVHD, but this subject need to be studied in different population.
细胞因子是决定移植结果的重要因素。细胞因子基因多态性可能会影响宿主产生细胞因子的能力。本研究旨在探讨白细胞介素-17（IL-17）基因多态性对造血干细胞移植结果的影响。
本研究共纳入60例骨髓移植受者。25例受者（41.66%）发生了移植物抗宿主病（GVHD）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估IL-17基因多态性，并通过酶联免疫吸附测定（ELISA）检测血清水平。
两组间IL-17（A/G）（rs3819025）基因型和等位基因的分布无显著差异。但是，与非GVHD组相比，GVHD组中IL-17（A/G，-197）GG基因型显著更高（P = 0.04）。有趣的是，根据GVHD严重程度对患者进行分层后，与（II-IV）级组相比（P = 0.05），GVHD（0-I）级组中IL-17（rs3819025）G等位基因仍显著更高。此外，根据患者性别进行分类后，IL-17 -197 GG基因型在男性患者中与非GVHD显著相关（P = 0.05）。GVHD组和非GVHD组之间的IL-17血清水平没有显示出任何显著差异。
结果表明，IL-17197 GG基因型、（rs3819025）的G等位基因及其血清水平对GVHD的严重程度具有预测价值。此外，IL-17 -197 GG基因型是GVHD发生的性别依赖性遗传危险因素，但这一问题需要在不同人群中进行研究。
