Kesić Srdjan, Nikolić Ljiljana, Savić Aleksandar G, Petković Branka, Spasić Sladjana Z
Department of Neurophysiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
Gen Physiol Biophys. 2014;33(3):335-44. doi: 10.4149/gpb_2014010. Epub 2014 Jun 26.
Aim of this study was to investigate the application of normalized mean of the empirical Higuchi fractal dimension (FD) distributions, as a new approach to analyze the spontaneous bioelectrical activity of garden snail (Helix pomatia) Br neuron. The effect of ouabain on modulation of Br neuron bursting activity before and after the exposure to 10 mT static magnetic field (SMF) was observed by analyzing the following parameters: action potential (AP), interspike interval (ISI) and interbursting interval (IBI) components. Normalized mean of the empirical FD distributions were formed for the following experimental conditions: Control 1, Ouabain 1, Control 2, SMF 2, ASMF 2, Control 3, SMF 3 and Ouabain ASMF 3. Our main results have shown that ouabain without SMF induced increase in participation of AP and a decrease in participation of IBI components compared to the first control condition. However, in the presence of 10 mT SMF, ouabain-induced changes of measured parameters of Br neuron activity were less pronounced compared to the third control condition. We have shown that normalized mean of the empirical FD distributions is a useful method for detecting the changes in AP, ISI, and IBI components of complex bursting activity in altered physiological states.
本研究的目的是探讨经验性Higuchi分形维数（FD）分布的归一化均值作为一种分析花园蜗牛（Helix pomatia）Br神经元自发生物电活动的新方法的应用。通过分析以下参数：动作电位（AP）、峰间期（ISI）和爆发间期（IBI）成分，观察哇巴因在暴露于10 mT静磁场（SMF）前后对Br神经元爆发活动调制的影响。针对以下实验条件形成了经验性FD分布的归一化均值：对照1、哇巴因1、对照2、SMF 2、交变SMF 2、对照3、SMF 3和哇巴因交变SMF 3。我们的主要结果表明，与第一个对照条件相比，无SMF的哇巴因诱导AP参与度增加，IBI成分参与度降低。然而，在存在10 mT SMF的情况下，与第三个对照条件相比，哇巴因诱导的Br神经元活动测量参数变化不太明显。我们已经表明，经验性FD分布的归一化均值是检测生理状态改变时复杂爆发活动的AP、ISI和IBI成分变化的有用方法。
