Wu Yuanjie, Wu Yuanbo, Zheng Shuguo, Wang Yufeng, Liu Xiaoli, Zhang Xiaojun, Lv Mingan
Anhui University of Chinese Medicine, Hefei 230038, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jun;34(6):771-6.
To establish a blood glucose fluctuation model in diabetic rats.
Male SD rats were randomly divided into 2 groups, namely normal group and diabetes group. Rat model of diabetes was established by single intraperitoneal injection of streptozotocin (STZ) and then divided into sustained high blood glucose group and blood glucose fluctuation group. Rat model of blood glucose fluctuation was established by subcutaneous injection with regular insulin or gavaging of glucose twice daily in diabetic rats. The general condition, body weight, daily blood glucose levels of 5 different times, daily average blood glucose (MBG), standard deviation of daily average blood glucose (SDBG), the maximum amplitude of glycemic excursions (LAGE), fasting serum insulin (FINS) and pancreatic tissue pathology were observed.
Rats in blood glucose fluctuation group and sustained high blood glucose group developed symptoms of polyphagia, polyuria and polydipsia. Though significant differences in body weight were observed at different time (P<0.01), no significant differences were found among the three groups (P>0.05). After 6 weeks of blood glucose fluctuation, MBG, SDBG and LAGE in blood glucose fluctuation group and sustained high blood glucose group were increased significantly than those in normal group (P<0.01), the level of FINS decreased markedly (P<0.05). SDBG and LAGE in blood glucose fluctuation group were higher than those in sustained high blood glucose group (P<0.01). Islet of diabetic rat became atrophy, irregular shape, sparse distribution, and decreased in number, and the changes were more obvious in blood glucose fluctuation group.
Rat model of blood glucose fluctuation can be successfully established by subcutaneous injection of regular insulin of gavage of glucose twice daily in diabetic rats.
建立糖尿病大鼠血糖波动模型。
雄性SD大鼠随机分为2组，即正常组和糖尿病组。采用单次腹腔注射链脲佐菌素（STZ）建立糖尿病大鼠模型，然后分为持续性高血糖组和血糖波动组。通过对糖尿病大鼠每日皮下注射正规胰岛素或灌胃葡萄糖2次建立血糖波动大鼠模型。观察大鼠的一般状况、体重、5个不同时间点的每日血糖水平、每日平均血糖（MBG）、每日平均血糖标准差（SDBG）、血糖波动最大幅度（LAGE）、空腹血清胰岛素（FINS）及胰腺组织病理学变化。
血糖波动组和持续性高血糖组大鼠均出现多食、多尿、多饮症状。不同时间点体重差异有统计学意义（P<0.01），但3组间差异无统计学意义（P>0.05）。血糖波动6周后，血糖波动组和持续性高血糖组的MBG、SDBG及LAGE均显著高于正常组（P<0.01），FINS水平明显降低（P<0.05）。血糖波动组的SDBG和LAGE高于持续性高血糖组（P<0.01）。糖尿病大鼠胰岛萎缩、形态不规则、分布稀疏、数量减少，血糖波动组变化更明显。
对糖尿病大鼠每日皮下注射正规胰岛素或灌胃葡萄糖2次可成功建立血糖波动大鼠模型。
