Zou Lin, Jiang Jianming, Yang Yong, Chen Changjun, Qu Dongbin
Department of Spinal Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jun;34(6):852-6.
To evaluate the structural characteristics and biocompatibility of a novel nano-Ta-Ti alloy rod for its potential application in internal fixation.
Ta coating of a Ti alloy rod with nano-Ta (tantalum) powder was performed using laser melting with symmetrical grooves repleted with nano-Ta powder along the whole length. The microstructure of the cross section of Ta-Ti alloy rod, pore diameter and components of the coating were analyzed using scanning electron microscopy. The influence of this nano-Ta-Ti alloy on proliferation and differentiation of MC3T3-E1 cells was evaluated by MTT cytotoxicity test and ALP activity test.
Under scanning electron microscope, the Ta-coating surface presented with a gross porous (200-300 µm) structure with dense fusion between Ta particles, and no new element was produced after laser melting. Biocompatibility evaluation showed that Ti alloys with and without Ta coating both promoted the proliferation of MC3T3-E1 cells, but the coated alloy showed better performance and obviously promoted the differentiation of the osteoblasts.
Alloying between Ta and Ti can be accomplished successfully by laser melting technique, and the alloy obtained has ideal surface structure and good biocompatibility.
评估一种新型纳米钽钛合金棒的结构特征和生物相容性，以探讨其在内部固定中的潜在应用。
采用激光熔覆技术，在钛合金棒表面涂覆纳米钽（Ta）粉末，沿棒的全长制备对称凹槽并填充纳米钽粉末。使用扫描电子显微镜分析钽钛合金棒横截面的微观结构、涂层的孔径和成分。通过MTT细胞毒性试验和碱性磷酸酶（ALP）活性试验评估这种纳米钽钛合金对MC3T3-E1细胞增殖和分化的影响。
在扫描电子显微镜下，钽涂层表面呈现出大孔（200 - 300 µm）结构，钽颗粒之间融合致密，激光熔覆后未产生新元素。生物相容性评估表明，有钽涂层和无钽涂层的钛合金均能促进MC3T3-E1细胞的增殖，但涂覆合金表现更佳，且明显促进了成骨细胞的分化。
通过激光熔覆技术可成功实现钽与钛的合金化，所得合金具有理想的表面结构和良好的生物相容性。
