Zhang Hongsheng, Kong Lingying, Cui Zhaolei, Du Wei, He Yihui, Yang Zhi, Wang Li, Chen Xiaoyan
Department of Pathology, Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China.
Department of Pathology, Fujian Provincial Hospital, Fuzhou, Fujian 350001, P.R. China.
Int J Mol Med. 2014 Sep;34(3):804-9. doi: 10.3892/ijmm.2014.1824. Epub 2014 Jun 27.
The role of the WW domain-containing oxidoreductase (WWOX) gene in multiple types of solid human cancers has been documented extensively. However, the functional role of WWOX in human multiple myeloma has not yet been fully elucidated. The present study aimed to investigate the effects of exogenous WWOX expression on the biological properties of U266 multiple myeloma cells, as well as the possible molecular mechanisms involved. In vitro experiments revealed that exogenous WWOX cDNA transfection resulted in marked growth arrest and the induction of apoptosis in the U266 multiple myeloma cells, accompanied by the activation of the intrinsic apoptotic pathway. Our data provide evidence that WWOX also plays a role as a tumor suppressor gene in multiple myeloma, possibly by suppressing cell proliferation and promoting apoptosis by triggering the intrinsic apoptotic pathway.
含WW结构域的氧化还原酶（WWOX）基因在多种人类实体癌中的作用已得到广泛记载。然而，WWOX在人类多发性骨髓瘤中的功能作用尚未完全阐明。本研究旨在探讨外源性WWOX表达对U266多发性骨髓瘤细胞生物学特性的影响以及可能涉及的分子机制。体外实验表明，外源性WWOX cDNA转染导致U266多发性骨髓瘤细胞明显生长停滞并诱导凋亡，同时伴有内源性凋亡途径的激活。我们的数据表明，WWOX在多发性骨髓瘤中也作为一种肿瘤抑制基因发挥作用，可能是通过触发内源性凋亡途径抑制细胞增殖并促进凋亡。
