The Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, 100730, China.
Arch Pharm Res. 2015;38(5):885-92. doi: 10.1007/s12272-014-0423-4. Epub 2014 Jun 28.
Rhein lysinate (RHL) is the salt of lysine and rhein and the objective of this study was to investigate the protection of RHL to liver in diabetic mice. The model of type 2 diabetes was established by high-fat diet and streptozotocin treatment. Malondialdehyde, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured using a spectrophotometer. Inflammatory factors (TNF-α and IL-6) and related proteins (ERK1/2 and SREBP-1c) were analyzed by Western blot. Tissue profile was determined by hematoxylin and eosin staining and accumulation of fat was examined by Nile red staining. The results indicated that plasma glucose levels of type 2 diabetic mice were over 13.9 mM. Compared with model group, plasma glucose levels were decreased, however insulin levels were increased in RHL (25 and 50 mg/kg)-treated group. Elevated plasma triglyceride and cholesterol were also markedly attenuated after RHL treatment. The activities of SOD and GSH-Px of livers were increased after RHL treatment. Livers of RHL-treated mice had more normal structure and less steatosis than that of diabetic mice. Moreover, RHL decreased the expression of TNF-α and IL-6 and the phosphorylation of SREBP-1c and ERK1/2. In conclusion, RHL has a noticeable hepatic protection in diabetic mice.
赖氨匹林（RHL）是赖氨酸和大黄酸的盐，本研究旨在探讨 RHL 对糖尿病小鼠肝脏的保护作用。采用高脂饮食联合链脲佐菌素（STZ）建立 2 型糖尿病模型。采用分光光度计法测定丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）。采用 Western blot 分析炎性因子（TNF-α和 IL-6）和相关蛋白（ERK1/2 和 SREBP-1c）。通过苏木精和伊红（H&E）染色确定组织形态，通过尼罗红染色检测脂肪堆积。结果表明，2 型糖尿病小鼠的血糖水平超过 13.9 mM。与模型组相比，RHL（25 和 50 mg/kg）治疗组的血糖水平降低，而胰岛素水平升高。RHL 治疗后，升高的血浆甘油三酯和胆固醇也明显降低。RHL 治疗后肝脏 SOD 和 GSH-Px 的活性增加。与糖尿病小鼠相比，RHL 治疗组的小鼠肝脏结构更正常，脂肪变性更少。此外，RHL 降低了 TNF-α和 IL-6 的表达以及 SREBP-1c 和 ERK1/2 的磷酸化。总之，RHL 对糖尿病小鼠的肝脏具有明显的保护作用。
