棕榈疫霉游动孢子分化过程中的加速离子通量。

Accelerated ion fluxes during differentiation in zoospores of Phytophthora palmivora.

作者信息

Iser J R, Griffith J M, Balson A, Grant B R

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.

出版信息

Cell Differ Dev. 1989 Jan;26(1):29-37. doi: 10.1016/0922-3371(89)90781-8.

DOI:10.1016/0922-3371(89)90781-8

PMID:2496900

Abstract

Zoospores of Phytophthora palmivora show increased fluxes of Na+ and Ca2+ 3-5 min after they have been stimulated to differentiate with pectin. Both spontaneous and pectin-induced encystment are reduced below pH 6 and accelerated above pH 7. The ionophores monensin and A23187 induce slow differentiation when added together, but not when added separately. Ethanol (0.5%) also induces slow differentiation. Amiloride and verapamil inhibit pectin-induced differentiation and also reduce the onset of the Na+ and Ca2+ flux. A requirement for Ca2+ for differentiation is confirmed, but a requirement for Na+ could not be demonstrated.

摘要

棕榈疫霉游动孢子在用果胶刺激其分化后3至5分钟，显示出Na⁺和Ca²⁺通量增加。在pH 6以下，自发和果胶诱导的包囊化均减少，而在pH 7以上则加速。离子载体莫能菌素和A23187一起添加时会诱导缓慢分化，但单独添加时则不会。乙醇（0.5%）也会诱导缓慢分化。氨氯吡脒和维拉帕米抑制果胶诱导的分化，也会减少Na⁺和Ca²⁺通量的起始。已证实分化需要Ca²⁺，但无法证明需要Na⁺。