Niu Huiyan, Zhang Yifei, Wu Baogang, Zhang Yi, Jiang Hongfang, He Ping
Department of Geriatrics, Shengjing Hospital, China Medical University, Shenyang 110004, P.R. China.
Oncol Rep. 2014 Sep;32(3):1087-93. doi: 10.3892/or.2014.3273. Epub 2014 Jun 20.
Lung cancer is the leading cause of cancer‑related mortality in humans. The prognosis for advanced lung cancer patients is extremely poor. Current standard care is rather ineffective for prolonging patient life while preserving satisfactory quality of life due to adverse side-effects. Matrine extracted from the traditional Chinese herbal plant Sophora flavescens was shown to induce cancer cell death in vitro. The aim of this study was to investigate the effect of matrine on the proliferation and apoptosis of lung cancer cells and the molecular basis of matrine-induced apoptosis. The results showed that matrine inhibited cell proliferation and induced apoptosis in lung cancer A549 and 95D cells in a dose- and time-dependent manner. The apoptotic effects of matrine on lung cancer cells appeared to act via the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K-Akt-mTOR) signaling pathway and downregulation of the expression of the inhibitor of apoptosis protein (IAP) family proteins. Matrine exerts its cancer-killing effect via promoting apoptosis in lung cancer cells and may be a useful adjuvant therapeutic scheme for treating advanced lung cancer patients.
肺癌是人类癌症相关死亡的主要原因。晚期肺癌患者的预后极差。由于存在不良副作用，目前的标准治疗在延长患者生命同时保持令人满意的生活质量方面相当无效。从传统中草药苦参中提取的苦参碱已显示出在体外诱导癌细胞死亡的作用。本研究的目的是探讨苦参碱对肺癌细胞增殖和凋亡的影响以及苦参碱诱导凋亡的分子基础。结果表明，苦参碱以剂量和时间依赖性方式抑制肺癌A549和95D细胞的增殖并诱导其凋亡。苦参碱对肺癌细胞的凋亡作用似乎是通过磷脂酰肌醇3-激酶/蛋白激酶B/雷帕霉素哺乳动物靶标（PI3K-Akt-mTOR）信号通路以及下调凋亡抑制蛋白（IAP）家族蛋白的表达来实现的。苦参碱通过促进肺癌细胞凋亡发挥其抗癌作用，可能是治疗晚期肺癌患者的一种有用的辅助治疗方案。
