Sun Xicai, Guo Limin, Wang Jingjing, Wang Huan, Liu Zhuofu, Liu Juan, Yu Huapeng, Hu Li, Li Han, Wang Dehui
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
Int J Pediatr Otorhinolaryngol. 2014 Aug;78(8):1232-8. doi: 10.1016/j.ijporl.2014.04.003. Epub 2014 Apr 12.
Although JNA is a benign neoplasm histopathologically, it has a propensity for locally destructive growth and remains a higher postoperative recurrence rate. The aim of this study was to analyze the expression and localization of MMP-9 in JNA using tissue microarray to elucidate its correlation with clinicopathological features and recurrence.
The expression of MMP-9 was assessed by immunohistochemistry in a tissue microarray from 70 patients with JNA and 10 control subjects. Correlation between the levels of MMP-9 expression and clinicopathologic variables, as well as tumor recurrence, were analyzed.
MMP-9 was detected in perivascular and extravascular less differentiated cells and stromal cells of patients with JNA but not in the matured vascular endothelial cells of these patients. The presence of MMP-9 expression in JNA was correlated with patient's age (p=0.001). Spearman correlation analysis suggested that high expression of MMP-9 in JNA had negative correlation with patient's age (r=-0.412, p<0.001). The recurrence rate in JNA patients with high MMP-9 expression was significantly higher than those with low MMP-9 expression (p=0.002). In multivariate and ROC curve analysis, MMP-9 was a good prognostic factor for tumor recurrence of JNA.
Higher MMP-9 expression is a poor prognostic factor for patients with JNA who have been surgically treated.
尽管鼻内翻性乳头状瘤（JNA）在组织病理学上是一种良性肿瘤，但它有局部侵袭性生长的倾向，术后复发率较高。本研究的目的是利用组织芯片分析JNA中基质金属蛋白酶-9（MMP-9）的表达和定位，以阐明其与临床病理特征和复发的相关性。
采用免疫组织化学方法评估70例JNA患者和10例对照者组织芯片中MMP-9的表达。分析MMP-9表达水平与临床病理变量以及肿瘤复发之间的相关性。
在JNA患者的血管周围和血管外低分化细胞及基质细胞中检测到MMP-9，但在这些患者的成熟血管内皮细胞中未检测到。JNA中MMP-9表达的存在与患者年龄相关（p = 0.001）。Spearman相关性分析表明，JNA中MMP-9的高表达与患者年龄呈负相关（r = -0.412，p < 0.001）。MMP-9高表达的JNA患者复发率显著高于MMP-9低表达患者（p = 0.002）。在多因素和ROC曲线分析中，MMP-9是JNA肿瘤复发的良好预后因素。
较高的MMP-9表达是接受手术治疗的JNA患者的不良预后因素。
