Zhu Dexing, Liu Fen, Dai Fang, Luo Xueqing, Hong Baozhi
Key Laboratory of Oral Medicine, School and Hospital of Stomatology, Guangzhou Medical University, Guangzhou 510140, China. Email:
Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Apr;49(4):216-9.
To investigate the expression of programmed death-1 (PD-1) and programmed death ligand-1(PD-L1) in peripheral T-lymphocytes from patients with chronic periodontitis and its significance and to clarify its role in the development of chronic periodontitis.
A total of 73 subjects were included in the study and divided into three groups, chronic periodontitis(30 cases), chronic gingivitis(25 cases) and 18 healthy controls. The peripheral blood was collected and PD-1/PD-L1 expression in the surface of CD(+)4 T lymphocytes and CD(+)8 T lymphocytes was examined by flow cytometry. Blood samples from 16 chronic periodontitis patients were collected at week 0 and 6 after initial therapy for 6 weeks and PD-1 and PD-L1 expression in the surface of CD(+)4 and CD(+)8 T lymphocytes was also determined by flow cytometry. The data were statistically analyzed.
The percentage of PD-1 expression in CD(+)4 and CD(+)8 T lymphocytes of chronic periodontitis group[(16.7 ± 5.5)%,(20.8 ± 5.1)%]and chronic gingivitis group[(14.2 ± 6.1)%,(14.5 ± 4.3)%]were higher than that of healthy controls[(9.5 ± 2.1)%, (8.1 ± 1.9)%](P < 0.05). The percentage of PD-L1 expression in CD(+)4 and CD(+)8 T lymphocytes of chronic periodontitis group[(24.2 ± 7.1)%,(15.3 ± 6.8)%]and chronic gingivitis group[(12.4 ± 6.0)%,(11.2 ± 5.5)%]were higher than that of healthy controls[(4.7 ± 1.2)%, (3.2 ± 2.3)%] (P < 0.05). The percentage of PD-1/PD-L1 expression in CD(+)4 T lymphocytes and CD(+)8 T lymphocytes of the chronic periodontitis group were significantly decreased after initial therapy(P < 0.05).
The expression of PD-1 and PD-L1 in peripheral CD(+)4 T lymphocytes and CD(+)8 T lymphocyte of chronic periodontitis patients was up-regulated and was associated with periodontal condition. The initial therapy reduced the expression of PD-1 and PD-L1.
探讨程序性死亡蛋白1（PD-1）和程序性死亡配体1（PD-L1）在慢性牙周炎患者外周血T淋巴细胞中的表达及其意义，阐明其在慢性牙周炎发生发展中的作用。
本研究共纳入73名受试者，分为三组，慢性牙周炎组（30例）、慢性牙龈炎组（25例）和18名健康对照者。采集外周血，采用流式细胞术检测CD4(+)T淋巴细胞和CD8(+)T淋巴细胞表面PD-1/PD-L1的表达。收集16例慢性牙周炎患者在初始治疗0周和6周后的血样，同样采用流式细胞术检测CD4(+)和CD8(+)T淋巴细胞表面PD-1和PD-L1的表达。对数据进行统计学分析。
慢性牙周炎组[（16.7±5.5）%，（20.8±5.1）%]和慢性牙龈炎组[（14.2±6.1）%，（14.5±4.3）%]CD4(+)和CD8(+)T淋巴细胞中PD-1表达百分比高于健康对照组[（9.5±2.1）%，（8.1±1.9）%]（P<0.05）。慢性牙周炎组[（24.2±7.1）%，（15.3±6.8）%]和慢性牙龈炎组[（12.4±6.0）%，（11.2±5.5）%]CD4(+)和CD8(+)T淋巴细胞中PD-L1表达百分比高于健康对照组[（4.7±1.2）%，（3.2±2.3）%]（P<0.05）。慢性牙周炎组CD4(+)T淋巴细胞和CD8(+)T淋巴细胞中PD-1/PD-L1表达百分比在初始治疗后显著降低（P<0.05）。
慢性牙周炎患者外周血CD4(+)T淋巴细胞和CD8(+)T淋巴细胞中PD-1和PD-L1表达上调，且与牙周状况相关。初始治疗降低了PD-1和PD-L1的表达。
