Gu Lisha, Mai Sui, Qi Yipin, Huang Qi, Ling Junqi
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China. Email:
Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Apr;49(4):224-8.
To investigate the functions of sodium tripolyphosphate (STTP) and polyacrylic acid (PAA) in the process of collagen biomimetic mineralization. This would allow future applications to other collagen matrices such as bone collagen or 3-D collagen scaffolds.
Glass cover slips and transmission electron microscopy (TEM) grids were coated with reconstituted typeIcollagen fibrils. Mineralization of the reconstituted collagens was demonstrated with scanning electron microscopy (SEM) and TEM using a Portland cement-containing resin composite and a phosphate-containing fluid in the presence of PAA and STTP. The rest were immersed in a biomimetic remineralization medium without PAA and/or STTP (control).
In the presence of PAA and STTP in the mineralization medium, intrafibrillar mineralization based on the non-classical crystallisation pathway could be identified. Mineral phases were evident within the collagen fibrils as early as 12 h after the initially-formed amorphous calcium phosphate nanoprecursors were transformed into apatite nanocrystals. Collagens at 72 h were heavily mineralized with periodically arranged intrafibrillar apatite platelets. Conversely, only large mineral spheres with no preferred association with collagen fibrils were observed in the absence of biomimetic analogues in the medium (control).
Intrafibrillar apatite deposition can be achieved via biomimetic mineralization system containing PAA and STTP when amorphous calcium phosphate precursor is stabilized.
研究三聚磷酸钠（STTP）和聚丙烯酸（PAA）在胶原仿生矿化过程中的作用。这将为未来应用于其他胶原基质（如骨胶原或3D胶原支架）提供可能。
用重组I型胶原纤维包被玻璃盖玻片和透射电子显微镜（TEM）网格。在PAA和STTP存在的情况下，使用含波特兰水泥的树脂复合材料和含磷酸盐的液体，通过扫描电子显微镜（SEM）和TEM对重组胶原进行矿化。其余样本浸入不含PAA和/或STTP的仿生再矿化介质中（对照）。
在矿化介质中存在PAA和STTP的情况下，可以识别基于非经典结晶途径的纤维内矿化。最早在最初形成的无定形磷酸钙纳米前体转化为磷灰石纳米晶体后12小时，胶原纤维内就出现了矿相。72小时时的胶原被大量矿化，纤维内有周期性排列的磷灰石板。相反，在介质中不存在仿生类似物的情况下（对照），只观察到与胶原纤维没有优先关联的大矿球。
当无定形磷酸钙前体稳定时，通过含有PAA和STTP的仿生矿化系统可以实现纤维内磷灰石沉积。
