大鼠急性脊髓损伤后纤连蛋白-1的时空表达
Temporospatial expression of fibulin-1 after acute spinal cord injury in rats.
作者信息
Xu Guanhua, Cui Ying, Wang Lingling, Zhang Jinlong, Shen Aiguo, Li Weidong, Bao Guofeng, Sun Yuyu, Cui Zhiming
出版信息
J Spinal Cord Med. 2015 Nov;38(6):709-16. doi: 10.1179/2045772314Y.0000000228. Epub 2014 Jun 26.
OBJECTIVE
Fibulin-1 is a matricellular protein that plays important roles in motility inhibition in a variety of cells and blocks the proliferation of cultured neural stem cells. The biological function of fibulin-1 in the spinal cord has not been fully elucidated.
METHODS
To clarify the expressions and possible functions of fibulin-1 in spinal cord injury (SCI), we performed an acute spinal cord contusion injury model in adult rats. Our work studied the temporospatial expression patterns of fibulin-1.
RESULTS
Western blot analysis revealed that fibulin-1 levels significantly increased 5 days after spinal cord contusion. Immunohistochemistry confirmed an increased number of fibulin-1 immunopositive cells about 2 mm from the lesion site. Moreover, double immunofluorescence labeling suggested that these changes were especially prominent in neurons and microglia.
CONCLUSION
These findings suggest that fibulin-1 may be involved in neuronal apoptosis and microglial activation after SCI.
目的
纤维连接蛋白-1是一种基质细胞蛋白，在多种细胞的运动抑制中起重要作用，并能阻断培养的神经干细胞的增殖。纤维连接蛋白-1在脊髓中的生物学功能尚未完全阐明。
方法
为了阐明纤维连接蛋白-1在脊髓损伤（SCI）中的表达及可能的功能，我们在成年大鼠中建立了急性脊髓挫伤损伤模型。我们的研究观察了纤维连接蛋白-1的时空表达模式。
结果
蛋白质免疫印迹分析显示，脊髓挫伤后5天纤维连接蛋白-1水平显著升高。免疫组织化学证实，在距损伤部位约2mm处，纤维连接蛋白-1免疫阳性细胞数量增加。此外，双重免疫荧光标记表明，这些变化在神经元和小胶质细胞中尤为明显。
结论
这些发现表明，纤维连接蛋白-1可能参与脊髓损伤后的神经元凋亡和小胶质细胞激活。
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