Kanwar Poonam, Sanyal Sibaji K, Tokas Indu, Yadav Akhilesh K, Pandey Amita, Kapoor Sanjay, Pandey Girdhar K
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi 110021, India.
Cell Calcium. 2014 Aug;56(2):81-95. doi: 10.1016/j.ceca.2014.05.003. Epub 2014 Jun 5.
Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein kinases designated as CBL-interacting protein kinases (CIPKs). The coordination of these two gene families defines complexity of the signaling networks in several stimulus-response-coupling during various environmental stresses. In Arabidopsis, both of these gene families have been extensively studied. To understand in-depth mechanistic interplay of CBL-CIPK mediated signaling pathways, expression analysis of entire set of CBL and CIPK genes in rice genome under three abiotic stresses (salt, cold and drought) and different developmental stages (3-vegetative stages and 11-reproductive stages) were done using microarray expression data. Interestingly, expression analysis showed that rice CBLs and CIPKs are not only involved in the abiotic stress but their significant role is also speculated in the developmental processes. Chromosomal localization of rice CBL and CIPK genes reveals that only OsCBL7 and OsCBL8 shows tandem duplication among CBLs whereas CIPKs were evolved by many tandem as well as segmental duplications. Duplicated OsCIPK genes showed variable expression pattern indicating the role of gene duplication in the extension and functional diversification of CIPK gene family in rice. Arabidopsis SOS3/CBL4 related genes in rice (OsCBL4, OsCBL5, OsCBL7 and OsCBL8) were employed for interaction studies with rice and Arabidopsis CIPKs. OsCBLs and OsCIPKs are not only found structurally similar but likely to be functionally equivalent to Arabidopsis CBLs and CIPKs genes since SOS3/CBL4 related OsCBLs interact with more or less similarly to rice and Arabidopsis CIPKs and exhibited an interaction pattern comparable with Arabidopsis SOS3/CBL4.
钙离子参与多种生理和发育途径。钙的重要作用之一是作为信号信使，调节植物中的信号转导。CBL（类钙调神经磷酸酶B蛋白）是一种钙传感器，它与一类被称为CBL互作蛋白激酶（CIPK）的丝氨酸 - 苏氨酸蛋白激酶特异性相互作用。这两个基因家族的协同作用定义了在各种环境胁迫下几个刺激 - 反应偶联中信号网络的复杂性。在拟南芥中，这两个基因家族都已得到广泛研究。为了深入了解CBL - CIPK介导的信号通路的机制相互作用，利用微阵列表达数据对水稻基因组中全套CBL和CIPK基因在三种非生物胁迫（盐、冷和干旱）以及不同发育阶段（3个营养阶段和11个生殖阶段）下进行了表达分析。有趣的是，表达分析表明水稻CBL和CIPK不仅参与非生物胁迫，而且推测它们在发育过程中也发挥重要作用。水稻CBL和CIPK基因的染色体定位表明，在CBL中只有OsCBL7和OsCBL8显示串联重复，而CIPK是通过许多串联以及片段重复进化而来的。重复的OsCIPK基因表现出可变的表达模式，表明基因重复在水稻CIPK基因家族的扩展和功能多样化中的作用。水稻中与拟南芥SOS3/CBL4相关的基因（OsCBL4、OsCBL5、OsCBL7和OsCBL8）用于与水稻和拟南芥CIPK的相互作用研究。OsCBL和OsCIPK不仅在结构上相似，而且在功能上可能与拟南芥CBL和CIPK基因等同，因为与SOS3/CBL4相关的OsCBL与水稻和拟南芥CIPK的相互作用或多或少相似，并且表现出与拟南芥SOS3/CBL4相当的相互作用模式。
