Köpke A K, Baier G, Wittmann-Liebold B
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Germany.
FEBS Lett. 1989 Apr 24;247(2):167-72. doi: 10.1016/0014-5793(89)81326-2.
An open reading frame upstream of the Methanococcus vannielii L12 gene has been detected. The beginning of this open reading frame agrees with the N-terminal region of a protein (MvaL10) which has been isolated from the 50 S ribosomal subunit of M. vannielii and sequenced. The length of this gene is 1008 nucleotides, coding for 336 amino acids. Excellent sequence similarities were found to the L10-like ribosomal proteins from Halobacterium halobium and man. The N-terminal part of the MvaL10 protein shows significant sequence similarities to the E. coli L10 protein. MvaL10 is more than twice as long as E. coli L10 but is of length similar to those of the homologous halobacterial and human proteins. Interestingly, the C-terminal region of MvaL10 shows exceptionally high similarity to the C-terminal sequence of the MvaL12 protein. This is not the case for the E. coli proteins but was also observed for the human, Halobacterium and Sulfolobus proteins.
已检测到万氏甲烷球菌L12基因上游的一个开放阅读框。该开放阅读框的起始位置与一种从万氏甲烷球菌50 S核糖体亚基中分离并测序的蛋白质（MvaL10）的N端区域一致。该基因长度为1008个核苷酸，编码336个氨基酸。发现它与嗜盐嗜盐菌和人类的L10样核糖体蛋白具有极好的序列相似性。MvaL10蛋白的N端部分与大肠杆菌L10蛋白具有显著的序列相似性。MvaL10的长度是大肠杆菌L10的两倍多，但与同源的嗜盐菌和人类蛋白质长度相似。有趣的是，MvaL10的C端区域与MvaL12蛋白的C端序列显示出异常高的相似性。大肠杆菌的蛋白质并非如此，但在人类、嗜盐菌和硫化叶菌的蛋白质中也观察到了这种情况。
