Flament S, Delacourte A
Unité INSERM no. 16, Laboratoire de Neurosciences, Faculté de Médecine, Lille, France.
FEBS Lett. 1989 Apr 24;247(2):213-6. doi: 10.1016/0014-5793(89)81337-7.
Tau proteins were detected in human brain using two polyclonal antibodies: anti-paired helical filaments and anti-human native tau proteins. Both antisera detected identically the normal set of tau proteins in control brains. Moreover they detected two abnormal tau variants of 64 and 69 kDa exclusively in brain areas showing neurofibrillary tangles and senile plaques. Tau 64 and 69 were abnormally phosphorylated as revealed by the decrease in their molecular mass observed after alkaline phosphatase treatment. Therefore, tau 64 and 69 are specific markers of the neurofibrillary degeneration of the Alzheimer type and might be useful tools for studying the first pathological events that lead to neuronal death.
使用两种多克隆抗体在人脑中检测到了tau蛋白：抗双螺旋丝抗体和抗人天然tau蛋白抗体。两种抗血清在对照脑中均能同样检测到正常的tau蛋白组。此外，它们仅在显示神经原纤维缠结和老年斑的脑区检测到两种异常的tau变体，分子量分别为64 kDa和69 kDa。碱性磷酸酶处理后观察到其分子量降低，表明tau 64和69发生了异常磷酸化。因此，tau 64和69是阿尔茨海默型神经原纤维变性的特异性标志物，可能是研究导致神经元死亡的早期病理事件的有用工具。
