在无细胞体系中，热休克时最后四个有义密码子对LacZ mRNA可翻译性的调控。
Regulation of LacZ mRNA translatability in a cell-free system at heat shock by the last four sense codons.
作者信息
Denisenko O N, Yarchuk O B
机构信息
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow region.
出版信息
FEBS Lett. 1989 Apr 24;247(2):251-4. doi: 10.1016/0014-5793(89)81346-8.
PMID:2497030
Abstract
Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell-free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli beta-galactosidase containing the last four sense codons of Drosophila hsp70 at its 3'-end was constructed. This mRNA is efficiently translated in a rabbit reticulocyte cell-free system at 43 degrees C.
摘要
在热休克条件下，非洲爪蟾正常mRNA在兔网织红细胞无细胞系统中的翻译被阻断，而热休克蛋白70（hsp70）mRNA仍可翻译。构建了在其3'端含有果蝇hsp70最后四个有义密码子的大肠杆菌β-半乳糖苷酶mRNA。该mRNA在43摄氏度的兔网织红细胞无细胞系统中能有效翻译。
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