中国野生 Reeves 麂（Muntiacus reevesi）中梨形虫的分子证据。
Molecular evidence for piroplasms in wild Reeves' muntjac (Muntiacus reevesi) in China.
作者信息
Yang Ji-fei, Li You-quan, Liu Zhi-jie, Liu Jun-long, Guan Gui-quan, Chen Ze, Luo Jian-xun, Wang Xiao-long, Yin Hong
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases MOA, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, PR China; Center of Conservation Medicine & Ecological Safety, Northeast Forestry University, 26 Xiangfang District, Harbin, 150040, Heilongjiang, PR China.
出版信息
Parasitol Int. 2014 Oct;63(5):713-6. doi: 10.1016/j.parint.2014.06.002. Epub 2014 Jun 24.
DNA from liver samples of 17 free-ranging wild Reeves' muntjac (Muntiacus reevesi) was used for PCR amplification of piropalsm 18S rRNA gene. Of 17 samples, 14 (82.4%) showed a specific PCR product which were cloned and sequenced. BLAST analysis of the sequences obtained showed similarities to Babesia sp., Theileria capreoli, Theileria uilenbergi and Theileria sp. BO302-SE. Phylogenetic analysis showed that the Babesia sp. detected in the present study was distantly separated from known Babesia species of wild and domestic animals. Six sequences showed 100% similarity to T. capreoli while five sequences were separated from all known Theileria species and constituted an independent clade with Theileria sp. BO302-SE derived from roe deer in Italy; two sequences were close to T. uilenbergi with 97% similarity. This is the first description of hemoparasite infection in free-ranging wild Reeves' muntjac in China. Our results indicate that wild Reeves' muntjac may play an important reservoir role for hemoparasites.
利用17只自由放养的野生中华麂（Muntiacus reevesi）肝脏样本的DNA对梨形虫18S rRNA基因进行PCR扩增。17个样本中，14个（82.4%）显示出特异性PCR产物，对其进行克隆和测序。对所得序列的BLAST分析表明，它们与巴贝斯虫属、绵羊泰勒虫、乌氏泰勒虫和泰勒虫属BO302-SE相似。系统发育分析表明，本研究中检测到的巴贝斯虫属与已知的野生动物和家畜巴贝斯虫物种相距较远。6个序列与绵羊泰勒虫显示出100%的相似性，而5个序列与所有已知泰勒虫物种分离，并与源自意大利狍的泰勒虫属BO302-SE构成一个独立分支；2个序列与乌氏泰勒虫接近，相似性为97%。这是中国自由放养的野生中华麂血液寄生虫感染的首次描述。我们的结果表明，野生中华麂可能对血液寄生虫起着重要的储存宿主作用。
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