Jansen P L, Hess F, Peters W H, Koenders E, Jerusalem C, Corstens F H
Division of Gastrointestinal and Liver Diseases, Academic Medical Center, Amsterdam, The Netherlands.
J Hepatol. 1989 Mar;8(2):192-200. doi: 10.1016/0168-8278(89)90007-x.
In this study auxiliary liver transplantation (ALT) has been tested as a means of correcting the UDP-glucuronyltransferase deficiency in Gunn rats and the UDP-glucuronyltransferase deficiency and impaired hepatobiliary bilirubin transport in double mutant rats. In both groups serum bilirubin normalized and remained low until the end of the study at 12 weeks after transplantation in 4 out of 6 rats. Excretion of 99mTc-HIDA in non-transplanted double mutants was considerably slower than in Gunn rats (kel 0.9 x 10(-3) versus 4.3 x 10(-3) s-1). HIDA excretion by transplants in double mutants and Gunn rats was about equal (kel 1.6 x 10(-3) and 1.1 x 10(-3) s-1). Experiments with bile duct-cannulated transplants showed that in double mutants bile flow, bile acid and bilirubin excretion was 2-4 times higher than in Gunn rats. This study shows that auxiliary liver transplants can conjugate and excrete bilirubin when one of these or both functions are lacking in the recipient's liver.
在本研究中，辅助性肝移植（ALT）已被作为一种纠正Gunn大鼠中尿苷二磷酸葡萄糖醛酸基转移酶缺乏以及双突变大鼠中尿苷二磷酸葡萄糖醛酸基转移酶缺乏和肝胆胆红素转运受损的方法进行了测试。在两组中，6只大鼠中有4只在移植后12周的研究结束时血清胆红素恢复正常并维持在低水平。未移植的双突变大鼠中99mTc-HIDA的排泄比Gunn大鼠慢得多（清除速率常数分别为0.9×10⁻³和4.3×10⁻³ s⁻¹）。双突变大鼠和Gunn大鼠中移植肝脏的HIDA排泄大致相等（清除速率常数分别为1.6×10⁻³和1.1×10⁻³ s⁻¹）。对胆管插管的移植肝脏进行的实验表明，双突变大鼠的胆汁流量、胆汁酸和胆红素排泄比Gunn大鼠高2至4倍。这项研究表明，当受体肝脏缺乏这些功能中的一种或两种时，辅助性肝移植可以结合并排泄胆红素。
