Kogut M H, Lange C
Department of Animal Sciences, Cook College, Rutgers, University of New Jersey, New Brunswick 08903.
J Interferon Res. 1989 Feb;9(1):67-77. doi: 10.1089/jir.1989.9.67.
Treatment of primary chicken kidney (CK) cultures with supernatants from concanavalin A-treated Eimeria tenella-immune avian splenic T cells significantly inhibited the invasion of the cells by sporozoites of E. tenella. Biochemical evaluation of this T-cell factor is consistent with the idea that this anti-Eimeria activity in the avian lymphokines is due to interferon-gamma (IFN-gamma). We then studied the effect of recombinant bovine and human IFN-gamma on the invasion of sporozoites of E. tenella in cultures of bovine and human cell lines, respectively. The initial infection of either the Madin-Darby bovine kidney cell line (MDBK) or the human larynx epidermoid carcinoma cell line (HEp-2) by the obligate intracellular protozoan parasite E. tenella was inhibited following a 24-h pretreatment with recombinant bovine or human IFN-gamma, respectively. The IFN-gamma-mediated inhibition was dose-dependent for both cell lines. Incubation of sporozoites of E. tenella with IFN-gamma alone before infection had no detectable inhibitory effect on cell invasion of either cell line by the parasite. In addition, the establishment of the antiinfective cellular state by IFN-gamma required at least a 16- to 24-h preincubation for significant inhibitory effects. These results are suggestive of a direct effect of the IFN-gamma on the host cells and not on the parasite themselves. Taken together these studies provide evidence for a role for IFN-gamma as a nonspecific mediator of host protection against coccidiosis.
用伴刀豆球蛋白A处理的柔嫩艾美耳球虫免疫禽类脾T细胞的上清液处理原代鸡肾（CK）培养物，可显著抑制柔嫩艾美耳球虫子孢子对细胞的侵袭。对这种T细胞因子的生化评估与以下观点一致，即禽类淋巴因子中的这种抗艾美耳球虫活性归因于γ干扰素（IFN-γ）。然后，我们分别研究了重组牛干扰素和人干扰素对牛和人细胞系培养物中柔嫩艾美耳球虫子孢子侵袭的影响。在用重组牛干扰素或人干扰素分别进行24小时预处理后，专性细胞内原生动物寄生虫柔嫩艾美耳球虫对Madin-Darby牛肾细胞系（MDBK）或人喉表皮样癌细胞系（HEp-2）的初始感染均受到抑制。两种细胞系中，IFN-γ介导的抑制作用均呈剂量依赖性。在感染前单独用IFN-γ孵育柔嫩艾美耳球虫子孢子，对寄生虫对任何一种细胞系的细胞侵袭均未检测到抑制作用。此外，IFN-γ建立抗感染细胞状态至少需要16至24小时的预孵育才能产生显著的抑制作用。这些结果表明IFN-γ对宿主细胞有直接作用，而不是对寄生虫本身有直接作用。综上所述，这些研究为IFN-γ作为宿主抗球虫病保护的非特异性介质的作用提供了证据。
