血清CD10与结直肠癌肝转移有关。

Serum CD10 is associated with liver metastasis in colorectal cancer.

作者信息

Sasaki Takamitsu, Kuniyasu Hiroki, Luo Yi, Fujiwara Rina, Kitayoshi Misaho, Tanabe Eriko, Kato Daisuke, Shinya Satoshi, Fujii Kiyomu, Ohmori Hitoshi, Yamashita Yuichi

机构信息

Department of Gastroenterological Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.

Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.

出版信息

J Surg Res. 2014 Dec;192(2):390-4. doi: 10.1016/j.jss.2014.05.071. Epub 2014 Jun 2.

DOI:10.1016/j.jss.2014.05.071

PMID:24972738

Abstract

INTRODUCTION

Colorectal cancer (CRC) is the third leading cause of cancer death in Japan. CD10 expression is closely associated with liver metastasis. In the present study, we explored the possibility of serum CD10 as a marker of liver metastasis in CRC.

METHODOLOGY

BALB/c mouse with subcutaneous tumor of syngeneic CT26 CRC cells were examined serum CD10. In 84 CRC patients and patients undergoing hemodialysis, serum CD10 was examined. CD10 concentration was measured by enzyme-linked immunosorbent assay.

RESULTS

In a mouse subcutaneous tumor model, serum CD10 correlated with the weight of the tumors. Serum CD10 was examined in 84 patients with CRC. The serum levels of CD10 were higher in patients with more advanced cancer stages. Patients with liver metastasis showed the highest levels of serum CD10 among all patients. Importantly, patients with high serum CD10 levels had metachronous liver metastasis. Healthy volunteers showed low levels of CD10; however, serum CD10 levels in patients undergoing hemodialysis showed levels as high as those with stage II and III CRC. A cutoff of serum CD10 set to >1000 pg/mL showed 70% sensitivity and 93% specificity for liver metastasis in CRC. This cutoff included all cases of metachronous liver metastasis.

CONCLUSIONS

With the exclusion of mimicking factors, serum CD10 levels might serve as a useful marker of synchronous and metachronous liver metastasis in CRC.

摘要

引言

在日本，结直肠癌（CRC）是癌症死亡的第三大主要原因。CD10表达与肝转移密切相关。在本研究中，我们探讨了血清CD10作为CRC肝转移标志物的可能性。

方法

对携带同基因CT26 CRC细胞皮下肿瘤的BALB/c小鼠检测血清CD10。对84例CRC患者和接受血液透析的患者检测血清CD10。通过酶联免疫吸附测定法测量CD10浓度。

结果

在小鼠皮下肿瘤模型中，血清CD10与肿瘤重量相关。对84例CRC患者检测血清CD10。癌症分期越晚的患者血清CD10水平越高。在所有患者中，肝转移患者的血清CD10水平最高。重要的是，血清CD10水平高的患者发生了异时性肝转移。健康志愿者的CD10水平较低；然而，接受血液透析的患者血清CD10水平与II期和III期CRC患者的水平一样高。将血清CD10的临界值设定为>1000 pg/mL时，对CRC肝转移的敏感性为70%，特异性为93%。该临界值包括所有异时性肝转移病例。