Xiong Dan, Pan Zhiming, Kang Xilong, Wang Jing, Song Li, Jiao Xinan
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu 225009, China; Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Res Vet Sci. 2014 Aug;97(1):43-5. doi: 10.1016/j.rvsc.2014.05.010. Epub 2014 Jun 11.
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-κB-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog.
Toll样受体5（TLR5）负责在脊椎动物中识别细菌鞭毛蛋白。在本研究中，我们克隆了麻鸭（Tadorna tadorna）玛雅品种的单外显子TLR5基因。TLR5开放阅读框长度为2580 bp，编码一个859个氨基酸的蛋白质。鸭TLR5的推测氨基酸序列由一个信号肽序列、11个富含亮氨酸的重复结构域、一个富含亮氨酸的重复C末端结构域、一个跨膜结构域和一个细胞内Toll-白细胞介素-1受体结构域组成。鸭TLR5基因在肺脏、骨髓、脾脏和肝脏中高表达；在肾脏、小肠、大肠和大脑中中度表达。构建了表达鸭TLR5的质粒并转染至HEK293T细胞中，通过间接免疫荧光测定法证实了其表达。用含有鸭TLR5和NF-κB荧光素酶的质粒转染的HEK293T细胞对鼠伤寒沙门氏菌的鞭毛蛋白有显著反应，表明它是一种功能性TLR5同源物。
