The Blight of Livestock and Poultry Research Laboratory, Institute of Animal Husbandry and Veterinary Medicine of Guizhou Academy of Agricultural Sciences, No. 1, Laolipo Nanming District, Guiyang, 550025, Guizhou, China.
College of Animal Science, Guizhou University, Guiyang, Guizhou, China.
Curr Microbiol. 2024 Aug 6;81(9):296. doi: 10.1007/s00284-024-03716-7.
Duck enteritis virus (DEV) may lead to vascular injury, gastrointestinal mucosal erosion, lymphoid organ injury, and Polyinosinic-polycytidylic acid (Poly I:C) has an antiviral effect by inducing low levels of interferon. The purpose of this study was to explore the pathogenesis of DEV-induced intestinal injury in ducks and to verify the therapeutic effects of different concentrations of Poly I:C. In this study, duck enteritis model was established by infecting healthy Pekin ducks with DEV. Duck intestinal tissues were extracted from normal control group, model group, and treatment group with different doses of Poly I:C. In vivo, HE and TUNEL staining were used to observe the morphological changes and apoptosis. In vitro, the proliferation and apoptosis of duck intestinal epithelial cells were evaluated by MTT assay, TUNEL staining, and flow cytometry. The results showed that Poly I:C protected ducks from DEV toxicity by improving intestinal morphology and inhibiting apoptosis. In addition, the antiviral effect of Poly I:C on DEV was found in a dose-dependent manner, with a more relatively obvious effect at a high dose of Poly I:C. All in all, these results demonstrated that Poly I:C played a vital role in the apoptosis induced by DEV in ducks and modest dose of Poly I:C treatment worked well and may provide important reference for the development of new antiviral drugs in the future.
鸭肠炎病毒（DEV）可能导致血管损伤、胃肠道黏膜侵蚀、淋巴器官损伤，而聚肌胞苷酸（Poly I:C）通过诱导低水平干扰素发挥抗病毒作用。本研究旨在探讨 DEV 诱导鸭肠损伤的发病机制，并验证不同浓度 Poly I:C 的治疗效果。本研究通过感染健康北京鸭 DEV 建立鸭肠炎模型。从正常对照组、模型组和不同剂量 Poly I:C 治疗组提取鸭肠组织。在体内，通过 HE 和 TUNEL 染色观察形态变化和细胞凋亡。在体外，通过 MTT 检测、TUNEL 染色和流式细胞术评估鸭肠上皮细胞的增殖和凋亡。结果表明，Poly I:C 通过改善肠道形态和抑制凋亡来保护鸭免受 DEV 毒性。此外，Poly I:C 对 DEV 的抗病毒作用呈剂量依赖性，高剂量 Poly I:C 的效果更为明显。总之，这些结果表明 Poly I:C 在 DEV 诱导的鸭凋亡中发挥了重要作用，适度剂量的 Poly I:C 治疗效果良好，可能为未来开发新型抗病毒药物提供重要参考。
