Tsai Sujune, Jhuang Yating, Spikings Emma, Sung Ping-Jyun, Lin Chiahsin
Department of Biotechnology, Mingdao University, 369 Wen-Hua Road, Peetow, Chang Hua 52345, Taiwan, ROC.
Institute of Marine Biotechnology, National Dong Hwa University, 2 Houwan Road, Checheng, Pingtung, Taiwan, ROC.
Tissue Cell. 2014 Aug;46(4):225-32. doi: 10.1016/j.tice.2014.05.002. Epub 2014 May 9.
The developmental oogenesis of gorgonian coral was investigated at the histological level. The objective of this study was to examine and improve the understanding of Junceella juncea oogenesis using ultrastructural methods, such as histological sectioning and transmission electron microscopy. At least three types of yolk materials were observed in this study: yolk body, lipid granules and cortical alveoli. Some of the complex yolk materials were encompassed by concentric or arched layers of smooth and rough endoplasmic reticulum and the Golgi complex in early stage oocytes. Different types of vesicles were found in both early and late stage oocytes and some granules could be seen inside the empty vesicles. This may be a possible method for elaborating complex yolk materials. Homogeneous yolks from different types of inclusions were abundant and the autosynthesis of yolk may be a major mechanism in J. juncea oocytes. This is the first report of the ultrastructural observation of oogenesis in gorgonian coral species using transmission electron microscopy. Our study obtained relatively detailed information at the ultrastructural level, and it provides an overview of the oocyte ultrastucture of the gorgonian coral J. juncea.
在组织学水平上研究了柳珊瑚的发育卵子发生。本研究的目的是使用组织切片和透射电子显微镜等超微结构方法，研究并加深对琼枝柳珊瑚卵子发生的理解。在本研究中观察到至少三种类型的卵黄物质：卵黄体、脂质颗粒和皮质小泡。在早期卵母细胞中，一些复杂的卵黄物质被光滑和粗糙内质网以及高尔基体的同心或拱形层所包围。在早期和晚期卵母细胞中均发现了不同类型的囊泡，并且在空囊泡内可见一些颗粒。这可能是一种形成复杂卵黄物质的可能方法。来自不同类型内含物的均匀卵黄丰富，卵黄的自身合成可能是琼枝柳珊瑚卵母细胞中的主要机制。这是首次使用透射电子显微镜对柳珊瑚物种卵子发生进行超微结构观察的报告。我们的研究在超微结构水平上获得了相对详细的信息，并提供了琼枝柳珊瑚卵母细胞超微结构的概述。
