使用基于荧光共振能量转移的荧光探针在体内监测半胱天冬酶激活。

In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe.

作者信息

Yamaguchi Yoshifumi, Kuranaga Erina, Nakajima Yu-Ichiro, Koto Akiko, Takemoto Kiwamu, Miura Masayuki

机构信息

Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan; PRESTO, JST, Tokyo, Japan.

Laboratory for Histogenetic Dynamics, RIKEN CDB, Kobe, Japan.

出版信息

Methods Enzymol. 2014;544:299-325. doi: 10.1016/B978-0-12-417158-9.00012-1.

DOI:10.1016/B978-0-12-417158-9.00012-1

PMID:24974295

Abstract

Caspases, which constitute a family of cysteine proteases, are highly conserved in multicellular organisms and function as a central player in apoptosis. The detection of apoptosis is intrinsically difficult because dying cells are rapidly removed from tissues by phagocytosis. Thus, the development of a method for detecting caspase activation is critical for the in vivo study of apoptosis. In this chapter, we describe a genetically encoded fluorescent probe for live imaging of caspase activation.

摘要

半胱天冬酶是一类半胱氨酸蛋白酶家族，在多细胞生物中高度保守，是细胞凋亡的核心参与者。由于死亡细胞会通过吞噬作用迅速从组织中清除，因此检测细胞凋亡本质上具有挑战性。因此，开发一种检测半胱天冬酶激活的方法对于细胞凋亡的体内研究至关重要。在本章中，我们描述了一种用于半胱天冬酶激活实时成像的基因编码荧光探针。