性腺类固醇对促性腺激素释放激素刺激滋养层细胞释放人绒毛膜促性腺激素的影响。

Effect of gonadal steroids on gonadotropin-releasing hormones stimulated human chorionic gonadotropin release by trophoblast cells.

作者信息

Iwashita M, Watanabe M, Adachi T, Ohira A, Shinozaki Y, Takeda Y, Sakamoto S

机构信息

Maternal and Perinatal Center, Tokyo Women's Medical College, Japan.

出版信息

Placenta. 1989 Jan-Feb;10(1):103-12. doi: 10.1016/0143-4004(89)90011-8.

DOI:10.1016/0143-4004(89)90011-8

PMID:2497453

Abstract

The effect of gonadal steroids on basal and GnRH-stimulated hCG release was studied using collagenase-dispersed trophoblast cells from early pregnancy. Both GnRH and a GnRH superagonist, Buserelin, stimulated hCG release with a similar dose dependency. Progesterone (0.1 to 10 micrograms/ml) inhibited GnRH-stimulated hCG release in a dose dependent manner as well as basal hCG release. Relatively high concentrations of estradiol (10 micrograms/ml) stimulated both basal and GnRH-mediated hCG release and antagonized the inhibitory effect of progesterone on hCG release at 1 micrograms/ml as well as RU486 (1 microgram/ml). These results indicate that progesterone has an important role in both basal and GnRH-mediated hCG regulatory system in the placenta.

摘要

利用来自妊娠早期经胶原酶分散的滋养层细胞，研究了性腺类固醇对基础和促性腺激素释放激素（GnRH）刺激的人绒毛膜促性腺激素（hCG）释放的影响。GnRH和一种GnRH超激动剂布舍瑞林均以相似的剂量依赖性刺激hCG释放。孕酮（0.1至10微克/毫升）以剂量依赖性方式抑制GnRH刺激的hCG释放以及基础hCG释放。相对高浓度的雌二醇（10微克/毫升）刺激基础和GnRH介导的hCG释放，并拮抗孕酮在1微克/毫升以及米非司酮（1微克/毫升）时对hCG释放的抑制作用。这些结果表明，孕酮在胎盘的基础和GnRH介导的hCG调节系统中均具有重要作用。