Ruampunpong Hathaiphan, Payungporn Sunchai, Samransamruajkit Rujipat, Pratheepamornkul Thitikarn, Theamboonlers Apiradee, Poovorawan Yong
Southeast Asian J Trop Med Public Health. 2014 May;45(3):610-21.
Human parainfluenza virus (HPIV) is a common cause of upper and lower respiratory illness in infants and young children. In order to classify the HPIV isolates circulating in the central part of Thailand, 650 samples obtained from the lower respiratory tract of patients from two hospital pediatric wards during 2010 to 2013, were analyzed for the presence and types of HPIVs by multiplex semi-nested PCR of hemagglutinin-neuraminidase (HN) gene. The results showed that 4.8% of the samples were positive for HPIV, among which 0.5%, 2.5% and 1.5% were positive for HPIV-1, HPIV-3, and HPIV-4, respectively, and none were positive for HPIV-2. A phylogenetic tree constructed from 31 HPIV HN gene sequences compared to those in GenBank showed greater than 80% identity to other reference strains. Prevalence of HPIV infection and phylogenetic characteristics of the circulating HPIVs may help explain the impact of HPIVs infection in Thai children.
人副流感病毒（HPIV）是婴幼儿上、下呼吸道疾病的常见病因。为了对泰国中部地区流行的HPIV分离株进行分类，对2010年至2013年期间从两家医院儿科病房的患者下呼吸道采集的650份样本，通过血凝素 - 神经氨酸酶（HN）基因的多重半巢式PCR分析HPIV的存在情况和类型。结果显示，4.8%的样本HPIV呈阳性，其中HPIV-1、HPIV-3和HPIV-4的阳性率分别为0.5%、2.5%和1.5%，HPIV-2均为阴性。与GenBank中的序列相比，由31个HPIV HN基因序列构建的系统发育树显示与其他参考菌株的同一性大于80%。HPIV感染的流行情况以及流行的HPIV的系统发育特征可能有助于解释HPIV感染对泰国儿童的影响。
