Shimizu Rika, Osawa Kayo, Shigemura Katsumi, Yoshida Hiroyuki, Fujiwara Miki, Iijima Yoshio, Fujisawa Masato, Shirakawa Toshiro
Southeast Asian J Trop Med Public Health. 2014 May;45(3):654-61.
More than 2,500 serovars of Salmonella species have been reported to date. A multiplex-PCR method was developed and evaluated for discriminating the four Salmonella enterica subsp enterica serovars, namely, S. Enteritidis, S. Typhimurium, S. Thompson and S. Infantis, most commonly isolated in Japan. Twenty-two serovars of 84 Salmonella strains and 7 species of non-Salmonella strains were evaluated using primer pairs specific for the detection of Salmonella spp. Multiplex PCR generated, with 100% specificity, the expected amplicon of 333, 413, 551 and 658 bp of S. Enteritidis, S. Infantis, S. Typhimurium, and S. Thompson, respectively, while an additional non-specific amplicon (about 1,000 bp) was observed for S. Infantis, but it had no practical impact in the bacterial detection. This multiplex PCR assay can be applied to identify and discriminate clinically significant strains of Salmonella serovars rapidly and accurately without the need for serological examination.
迄今为止，已报道了2500多种沙门氏菌血清型。开发并评估了一种多重PCR方法，用于区分在日本最常分离出的四种肠炎沙门氏菌亚种肠炎血清型，即肠炎沙门氏菌、鼠伤寒沙门氏菌、汤普森沙门氏菌和婴儿沙门氏菌。使用特异性检测沙门氏菌属的引物对，对84株沙门氏菌的22个血清型和7种非沙门氏菌菌株进行了评估。多重PCR分别产生了肠炎沙门氏菌、婴儿沙门氏菌、鼠伤寒沙门氏菌和汤普森沙门氏菌预期的333、413、551和658 bp扩增子，特异性为100%，而婴儿沙门氏菌还观察到一个额外的非特异性扩增子（约1000 bp），但这对细菌检测没有实际影响。这种多重PCR检测方法可用于快速、准确地鉴定和区分具有临床意义的沙门氏菌血清型菌株，而无需进行血清学检查。
