Institute of Sustainable Plant Protection, National Council of Research, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Department of Agricultural, Ambient and Agro-Food Sciences, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
Environ Pollut. 2014 Oct;193:1-5. doi: 10.1016/j.envpol.2014.06.001. Epub 2014 Jun 26.
Ethylenediurea (EDU) is the most common chemical used to prevent ozone (O3) injury on vegetation. Despite considerable research, its mode of action remains elusive and gene expression has not been studied. Transcripts of major antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase) were measured for the first time in a model plant (Phaseolus vulgaris cv S156) after short-term O3 exposure (0 or 90 ppb, 5 h/d, 4 days) and a single spray with EDU (0 or 300 ppm). Visible, physiological and biochemical parameters were assessed as indices of O3-induced stress. In O3-exposed EDU-protected plants, levels of transcript, enzyme activity, H2O2 accumulation, gas exchange and foliar visible injury were similar to those in control plants. These results suggest that EDU may halt the O3-induced ROS generation within 24 h from the exposure, and thus the downstream cascade mechanisms leading to increased H2O2 production, impaired gas exchange, and occurrence of leaf lesions.
乙二脒脲（EDU）是预防植被臭氧（O3）伤害最常用的化学物质。尽管进行了大量研究，但它的作用模式仍不清楚，基因表达也尚未研究。在模式植物（菜豆 cv S156）中，首次在短期 O3 暴露（0 或 90 ppb，每天 5 小时，持续 4 天）和单次喷洒 EDU（0 或 300 ppm）后测量了主要抗氧化酶（过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶）的转录物。将可见的、生理的和生化参数评估为 O3 诱导应激的指标。在 O3 暴露的 EDU 保护植物中，转录物水平、酶活性、H2O2 积累、气体交换和叶片可见损伤与对照植物相似。这些结果表明，EDU 可能在暴露后 24 小时内阻止 O3 诱导的 ROS 生成，从而阻止导致 H2O2 产生增加、气体交换受损和叶片病变发生的下游级联机制。
