Liu Jiajia, Wu Haimei, Zheng Feng, Liu Wenyuan, Feng Feng, Xie Ning
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.
J Sep Sci. 2014 Sep;37(18):2513-22. doi: 10.1002/jssc.201400379. Epub 2014 Aug 1.
Meconopsis horridula Hook.f. Thoms has been used as a traditional Tibetan medicine to clear away heat, relieve pain, and mobilize static blood. In this study, a reliable method based on high-performance liquid chromatography with diode array detection and electrospray ionization quadrupole time-of-flight tandem mass spectrometry was established for the identification of components in this herb. A total of 40 compounds (including 17 flavonoids, 15 alkaloids, and eight phenylpropanoids) were identified or tentatively identified. Among them, 17 components were identified in the herb for the first time. Compound 39 appears to be a novel compound, which is confirmed as 3-(kaempferol-8-yl)-2,3-epoxyflavanone by NMR spectroscopy and mass spectrometry. Moreover, seven major constituents were simultaneously quantified by the developed high-performance liquid chromatography with tandem triple-quadrupole mass spectrometry method. The quantitative method was validated and quality parameters were established. The study provides a comprehensive approach for understanding this herbal medicine.
藏药多刺绿绒蒿（Meconopsis horridula Hook.f. Thoms）具有清热、止痛、活血化瘀的功效。本研究建立了一种基于高效液相色谱-二极管阵列检测-电喷雾电离四极杆飞行时间串联质谱的可靠方法，用于鉴定该草药中的成分。共鉴定或初步鉴定出40种化合物（包括17种黄酮类化合物、15种生物碱和8种苯丙素类化合物）。其中，有17种成分首次在该草药中被鉴定出来。化合物39似乎是一种新化合物，通过核磁共振光谱和质谱确认为3-(山奈酚-8-基)-2,3-环氧黄烷酮。此外，采用所建立的高效液相色谱-串联三重四极杆质谱法同时对7种主要成分进行了定量分析。对该定量方法进行了验证并建立了质量参数。该研究为深入了解这种草药提供了一种全面的方法。
