Stern M L, Holsapple M P, McCay J A, Munson A E
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
Toxicol Ind Health. 1989 Jan;5(1):31-43. doi: 10.1177/074823378900500103.
Glutaraldehyde has a wide spectrum of uses which can result in dermal contact with the agent. The low number of reports of hypersensitive reactions to glutaraldehyde indicates a low incidence of sensitization. This paper describes the contact hypersensitivity response to glutaraldehyde in the guinea pig and the mouse. Female albino Hartley strain guinea pigs and female B6C3F1 mice were sensitized with 0.3, 1.0 and 3.0% glutaraldehyde and challenged with 10% glutaraldehyde. Doses of glutaraldehyde were selected from assays for primary irritancy. Guinea pigs received 100 microliters by direct dermal application, for 14 consecutive days, and mice received 20 microliters by direct dermal application, for 5 or 14 consecutive days, to sites prepared by shaving and dermabrading. Rest periods were 7 or 14 days for guinea pigs and 4 or 7 days for mice. Measurement of the contact hypersensitivity response in guinea pigs was both visual evaluation (scoring) at 24 and 48 hours following challenge and radioisotopic assay at 48 hours, and in mice by radioisotopic assay 48 hours after challenge. Both guinea pigs and mice demonstrated dose-dependent contact hypersensitivity responses to glutaraldehyde. The radioisotopic assay appeared to be more sensitive than visual evaluation in detecting contact allergic hypersensitivity to glutaraldehyde.
戊二醛有广泛的用途，这可能导致其与皮肤接触。关于戊二醛过敏反应的报告数量较少，表明致敏发生率较低。本文描述了豚鼠和小鼠对戊二醛的接触性超敏反应。用0.3%、1.0%和3.0%的戊二醛对雌性白化哈特利品系豚鼠和雌性B6C3F1小鼠进行致敏，并用10%的戊二醛进行激发。戊二醛的剂量选自原发性刺激性试验。豚鼠通过直接皮肤涂抹接受100微升，连续14天，小鼠通过直接皮肤涂抹接受20微升，连续5天或14天，涂抹于通过剃毛和磨皮准备好的部位。豚鼠的休息期为7天或14天，小鼠的休息期为4天或7天。豚鼠接触性超敏反应的测量方法是在激发后24小时和48小时进行视觉评估（评分）以及在48小时进行放射性同位素测定，小鼠则在激发后48小时进行放射性同位素测定。豚鼠和小鼠均表现出对戊二醛的剂量依赖性接触性超敏反应。在检测对戊二醛的接触性过敏性超敏反应方面，放射性同位素测定似乎比视觉评估更敏感。
