逼尿肌过度活动大鼠贮液囊钙激活氯通道的变化。

Changes in store-operated calcium channels in rat bladders with detrusor overactivity.

机构信息

Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.

出版信息

Urology. 2014 Aug;84(2):491.e1-6. doi: 10.1016/j.urology.2014.05.007. Epub 2014 Jun 26.

DOI:10.1016/j.urology.2014.05.007

PMID:24975715

Abstract

OBJECTIVE

To investigate the regulation of intracellular store-operated calcium channels (SOCCs) in detrusor overactivity (DO) during detrusor function changes in Sprague-Dawley rats.

METHODS

Sixty female Sprague-Dawley rats were randomized into control and DO groups. The contraction of the smooth muscle of the bladder was evaluated in vivo using smooth muscle strips. Changes in intracellular calcium ions were observed using confocal microscopy with preload fluo-4 AM, the SOCC agonist cyclopiazonic acid (CPA; 10 μM) and inhibitor SKF-96365 (10 μM). Cell currents were recorded with the whole-cell patch-clamp technique.

RESULTS

The in vitro frequencies of bladder smooth muscle contraction were significantly different (P <.05) between the DO and control groups, and the amplitudes were not significantly different (P >.05). The changes in intracellular calcium ions and current density were significantly different between the 2 groups (P <.05).

CONCLUSION

SOCCs were involved in DO and caused variations in muscle contraction.

摘要

目的

研究在逼尿肌过度活动（DO）期间逼尿肌功能变化中细胞内储存操纵钙通道（SOCCs）的调节。

方法

将 60 只雌性 Sprague-Dawley 大鼠随机分为对照组和 DO 组。使用平滑肌条在体内评估膀胱平滑肌的收缩。使用预先加载 fluo-4 AM 的共聚焦显微镜观察细胞内钙离子的变化，使用 SOCC 激动剂环匹阿尼酸（CPA；10 μM）和抑制剂 SKF-96365（10 μM）。使用全细胞膜片钳技术记录细胞电流。