Vairo S, Saey V, Bombardi C, Ducatelle R, Nauwynck H
Laboratory of Virology, Department of Virology, Parasitology and ImmunologyGhent University, B-9820 Merelbeke, Belgium.
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
J Comp Pathol. 2014 Aug-Oct;151(2-3):238-43. doi: 10.1016/j.jcpa.2014.04.008. Epub 2014 Jun 27.
In the last two decades, outbreaks of equine viral arteritis (EVA) have been reported in Europe, but little is known about these European isolates of equine arteritis virus (EAV). EAV European strain (08P178, EU-1 clade) isolated from one of these recent outbreaks is able to cause clinical signs on experimental infection. The aim of the present study was to investigate the microscopical lesions induced by this isolate after experimental infection of ponies. Animals were killed at 3, 7, 14 and 28 days post infection (dpi). At 3 dpi, lesions were essentially restricted to the respiratory tract and intestines and were characterized by mild multifocal epithelial degeneration and associated mononuclear cell infiltration. Lesions were more severe at 7 dpi and by 14 dpi, respiratory lesions were even more severe and lymphoplasmacytic infiltrates extended to other organs. At 28 dpi, lesions were still present in the viscera. In all specimens the most prominent histological change was intraepithelial, subepithelial and perivascular lymphoplasmacytic infiltration, ranging from mild and multifocal to extensive and diffuse. No signs of arterial damage such as infarcts, haemorrhages or necrosis were found. In conclusion, infection of naïve animals with the European 08P178 strain of EAV is associated with inflammation, but not arteritis.
在过去二十年中，欧洲已报告了马病毒性动脉炎（EVA）的疫情，但对这些欧洲分离的马动脉炎病毒（EAV）知之甚少。从最近一次疫情中分离出的EAV欧洲毒株（08P178，EU-1分支）在实验感染时能够引发临床症状。本研究的目的是调查该毒株在实验感染小马后所引起的微观病变。在感染后3、7、14和28天处死动物。在感染后3天，病变主要局限于呼吸道和肠道，其特征为轻度多灶性上皮变性及相关单核细胞浸润。在感染后7天病变更为严重，到感染后14天，呼吸道病变更加严重，淋巴细胞和浆细胞浸润扩展至其他器官。在感染后28天，内脏中仍存在病变。在所有标本中，最显著的组织学变化是上皮内、上皮下和血管周围淋巴细胞和浆细胞浸润，范围从轻度多灶性到广泛弥漫性。未发现梗死、出血或坏死等动脉损伤迹象。总之，用EAV欧洲08P178毒株感染未接触过该病毒的动物会引发炎症，但不会引发动脉炎。
