Bahadori Motahareh, Zarei Saeed, Zarnani Amir Hassan, Zarei Omid, Idali Farah, Hadavi Reza, Jeddi-Tehrani Mahmood
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran, e-mail:
Iran J Immunol. 2014 Jun;11(2):97-104.
Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM).
To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls.
The frequencies of IL-10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls.
These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.
促炎和抗炎细胞因子及其基因多态性被认为与复发性流产（RM）的发病机制有关。
在伊朗女性中，研究RM与细胞因子基因白细胞介素10（IL-10，-592 A/C、-819 C/T、-1082 A/G）、IL-6（-174 C/G）和IL-17（-197 G/A）的五种多态性之间的关联。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，测定85例RM女性和104例健康对照者中IL-6、IL-10和IL-17基因多态性的频率。
RM女性中IL-10启动子基因多态性（-592 A/C和-819 C/T）的频率显著高于对照组（p = 0.003）。然而，RM女性和对照组之间IL-6（-174 C/G）、IL-10（-1082 A/G）和IL-17（-197 G/A）多态性频率未观察到统计学显著差异。
这些结果表明，IL-10基因多态性筛查可能与RM患者有一定关联，这一观点需要进一步研究。
