State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
J Genet Genomics. 2014 Jun 20;41(6):317-25. doi: 10.1016/j.jgg.2014.04.005. Epub 2014 May 15.
The highly conserved Notch signaling is precisely regulated at different steps in a series of developmental events. However, little is known about the regulation of Notch receptor at transcriptional level. Here, we demonstrate that dBrms1 is involved in regulating Notch signaling in Drosophila wing. We show that knockdown of dBrms1 by RNA interference (RNAi) in wing disc suppresses the expression of Notch signaling target genes wingless (wg), cut and Enhancer of split m8 [E(spl)m8]. Consistently, the levels of Wg and Cut are reduced in the dBrms1 mutant clones. Importantly, loss of dBrms1 leads to significant reduction of Notch proteins. Furthermore, depletion of dBrms1 results in apparent downregulation of Notch transcription in the wing disc. Moreover, we find that dBrms1 is functionally conserved with human Breast cancer metastasis suppressor 1 like (hBRMS1L) in the modulation of Notch signaling. Taken together, our data provide important insights into the biological function of dBrms1 in regulating Notch signaling.
高度保守的 Notch 信号在一系列发育事件的不同步骤中被精确调控。然而，关于 Notch 受体在转录水平的调控知之甚少。在这里，我们证明了 dBrms1 参与了果蝇翅膀中 Notch 信号的调控。我们发现，通过 RNA 干扰 (RNAi) 在翅膀盘中敲低 dBrms1 会抑制 Notch 信号靶基因 wingless (wg)、cut 和 Enhancer of split m8 [E(spl)m8] 的表达。一致地，dBrms1 突变克隆中 Wg 和 Cut 的水平降低。重要的是，dBrms1 的缺失导致 Notch 蛋白的显著减少。此外，dBrms1 的耗竭导致翅膀盘中 Notch 转录的明显下调。此外，我们发现 dBrms1 在调节 Notch 信号方面与人类乳腺癌转移抑制因子 1 样 (hBRMS1L) 具有功能保守性。总之，我们的数据为 dBrms1 在调节 Notch 信号中的生物学功能提供了重要的见解。
