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钙补充剂增加了产卵季节末期河鳟 Salvelinus fontinalis 精子的激活。

Calcium ion supplementation increases brook trout Salvelinus fontinalis spermatozoa activation at the end of the spawning season.

机构信息

Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic.

出版信息

J Fish Biol. 2014 Sep;85(3):933-7. doi: 10.1111/jfb.12445. Epub 2014 Jun 27.

Abstract

The role of environmental ion composition and osmolality in calcium ion (Ca(2+) ) signalling of spermatozoa activation over the course of the spawning period of brook trout Salvelinus fontinalis was investigated. Motility at the end of spawning was low (mean ± s.d. of 5 ± 2% motile spermatozoa with curvilinear velocity of 25 ± 8 µm s(-1) ). Addition of 10 mM Ca(2+) to the activation medium resulted in values similar to those recorded during the middle of the spawning period (mean ± s.d. of 95 ± 6% motile spermatozoa with curvilinear velocity of 130 ± 15 µm s(-1) ).

摘要

研究了在布鲁克鲑鱼产卵期过程中,环境离子组成和渗透压在精子激活过程中钙离子(Ca(2+))信号传导中的作用。产卵期末的游动性较低(平均±标准偏差为 5±2%的运动精子,曲线速度为 25±8µm/s)。向激活介质中添加 10mM Ca(2+),结果与产卵中期记录的值相似(平均±标准偏差为 95±6%的运动精子,曲线速度为 130±15µm/s)。

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