Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic.
J Fish Biol. 2014 Sep;85(3):933-7. doi: 10.1111/jfb.12445. Epub 2014 Jun 27.
The role of environmental ion composition and osmolality in calcium ion (Ca(2+) ) signalling of spermatozoa activation over the course of the spawning period of brook trout Salvelinus fontinalis was investigated. Motility at the end of spawning was low (mean ± s.d. of 5 ± 2% motile spermatozoa with curvilinear velocity of 25 ± 8 µm s(-1) ). Addition of 10 mM Ca(2+) to the activation medium resulted in values similar to those recorded during the middle of the spawning period (mean ± s.d. of 95 ± 6% motile spermatozoa with curvilinear velocity of 130 ± 15 µm s(-1) ).
研究了在布鲁克鲑鱼产卵期过程中,环境离子组成和渗透压在精子激活过程中钙离子(Ca(2+))信号传导中的作用。产卵期末的游动性较低(平均±标准偏差为 5±2%的运动精子,曲线速度为 25±8µm/s)。向激活介质中添加 10mM Ca(2+),结果与产卵中期记录的值相似(平均±标准偏差为 95±6%的运动精子,曲线速度为 130±15µm/s)。