Nelson Kevin, Bobba Christopher, Ghadiali Samir, Hayes Don, Black Sylvester M, Whitson Bryan A
Kevin Nelson, Christopher Bobba, Samir Ghadiali, Department Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States.
World J Exp Med. 2014 May 20;4(2):7-15. doi: 10.5493/wjem.v4.i2.7.
Ex vivo lung perfusion (EVLP) is a powerful experimental model for isolated lung research. EVLP allows for the lungs to be manipulated and characterized in an external environment so that the effect of specific ventilation/perfusion variables can be studied independent of other confounding physiologic contributions. At the same time, EVLP allows for normal organ level function and real-time monitoring of pulmonary physiology and mechanics. As a result, this technique provides unique advantages over in vivo and in vitro models. Small and large animal models of EVLP have been developed and each of these models has their strengths and weaknesses. In this manuscript, we provide insight into the relative strengths of each model and describe how the development of advanced EVLP protocols is leading to a novel experimental platform that can be used to answer critical questions in pulmonary physiology and transplant medicine.
体外肺灌注（EVLP）是用于离体肺研究的强大实验模型。EVLP可使肺在外部环境中得到操控和特性分析，从而能够独立于其他混杂的生理因素来研究特定通气/灌注变量的影响。同时，EVLP可实现正常器官水平的功能以及对肺生理和力学的实时监测。因此，该技术相对于体内和体外模型具有独特优势。已经开发出了小型和大型动物的EVLP模型，且每种模型都有其优缺点。在本手稿中，我们深入探讨了每种模型的相对优势，并描述了先进的EVLP方案的发展如何促成一个可用于解答肺生理学和移植医学关键问题的新型实验平台。
