盘基网柄菌体内和体外的丙酮酸氧化作用

Pyruvate oxidation in vivo and in vitro in Dictyostelium discoideum.

作者信息

Butler M H, Wright B E

机构信息

Microbiology Department, University of Montana, Missoula 59812.

出版信息

Biochim Biophys Acta. 1989 May 31;991(2):337-9. doi: 10.1016/0304-4165(89)90125-6.

DOI:10.1016/0304-4165(89)90125-6

PMID:2497786

Abstract

The in vivo rate of the reaction catalyzed by the pyruvate dehydrogenase complex in Dictyostelium discoideum was measured by exposing cells to [1-14C]alanine and relating the rate of 14CO2 evolution to the specific radioactivity of the isolated [1-14C]pyruvate. The rate obtained, 0.33 mumol/min per ml packed cell volume, was very similar to that determined by independent calculations based upon the rate of utilization of endogenous protein as an energy source in this system.

摘要

通过将盘基网柄菌细胞暴露于[1-¹⁴C]丙氨酸，并将¹⁴CO₂的释放速率与分离出的[1-¹⁴C]丙酮酸的比放射性相关联，来测定盘基网柄菌中丙酮酸脱氢酶复合体催化反应的体内速率。得到的速率为每毫升压实细胞体积0.33微摩尔/分钟，这与基于该系统中内源性蛋白质作为能量来源的利用速率进行的独立计算所确定的速率非常相似。